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• 基金项目：

• 陈健斌
• 中国科学院微生物研究所

• 韩树琴
• 中国科学院微生物研究所

• 黄武华
• 中国科学院微生物研究所

• 林力
• 中国科学院微生物研究所

• 王先彬
• 中国科学院微生物研究所

• 鲜海军
• 中国科学院微生物研究所

• 摘要：从7个含酚废水生化处理厂的活性污泥或生物膜中分离出39株降解苯酚的酵母,它们均能以苯酚作为生长的唯一碳源,24小时内可将500ppm的苯酚去除90%以上。经鉴定,39株酵母分别属于两属三个种:热带假丝酵母(Candida tropicalis),皮状丝孢酵母(Trichosporon cutaneum)和乳头状丝孢酵母(Trichosporon capitatum)。不经诱导,在44-48小时内,热带假丝酵母314号最高生长的酚浓度和耐酚浓度分别约为1000ppm和1150ppm;皮状丝孢酵母25号约为800ppm和1000ppm;乳头状丝孢酵母115号约为750ppm和1450PPm。三种酵母在苯酚中生长的最适温度依次为30℃、25-30℃和25-36℃。它们生长的最适pH范围均为4.8-7.0。氰化物对这三种酵母的生长有明显的抑制作用。对含酚废水生化处理过程中的优势酵母等向题进行了讨论。

• Abstract：39 strains of phenol-degrading yeasts were isolated from activated sludge and biological film in 7 wastewater treatment plants which contained phenol. All these strains can utilize phenol as the sole carbon source for growth, and remove phenol at 600 ppm more than 90% within 24 hours. They were identified as Candida tropiealis, TrieTiasporon cutaneum and Triehosporon eapitatum. Without being induced, the strain can have concentrations of phenol causing both maximum growth and phenol-resistant growth at about 1000 Ppm and 1160 ppm for C. tropiealis; respectively within 24-48 hours about 800 ppm and 1000 ppm for T. cutaneum; and about 750 ppm and 1500 ppm for T. capitatum. The optimum temperature for growth on phenol is 30℃ for C. tropiealis; 25-30℃ for T. cutaneum; and 25-36℃ for T. capitatum. The optimum pH for three of them lies in the range of 4.8-7.0. The phenol-degrading yeasts show obviously inhibitive action on cyanide. The problem of dominant species of yeasts in the biological treatment of wastewater containing phenol was discussed.

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