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• 王选良
• 大连轻工业学院

• 摘要：本文研究了一株新菌种--井式假单胞杆菌(Pseudomonas wellantypicum)的培养方法.并对由该培养物提取的脱色酶的活性作了试验.脱色速度在12-24小时之间表现最大;脱色最适宜pH为7.0;脱色最适宜温度为45℃;70℃处理后失去脱色活性;半胱氨酸对脱色活性有一定的激活作用.该脱色酶制剂同时具有琼脂水解活性、蛋白质水解活性、淀粉水解活性、木聚糖水解活性和羧甲基纤维素水解活性.

• Abstract：A new species of agar-digesting bacteria (Pseudomonas wellanty picum) in shaking culture was studied with a synthetic medium composed of agar 60g, NaNO₃ 10g, KH₂PO₄ 1.5g, MgSO₄·7H₂O 0.7g, tap water 1000ml at pH 7.0. The crude enzymatic preparation of melanoidin decolorase was fractionated by ammonium sulfate precipitation from the cultural broth. The enzymatic activity of decolorization or agar-digestion was the highest at 45℃ and pH7.0. This enzyme also showed hydrolytic activity towards agar, protein, xylan, starch and carboxymethyl cellulose.

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