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• 基金项目：

• 王春生
• 中国科学院武汉病毒研究所

• 罗清修
• 中国科学院武汉病毒研究所

• 陆娟子
• 中国科学院武汉病毒研究所

• 孙国萍
• 中国科学院武汉病毒研究所

• 王淑君
• 中国科学院武汉病毒研究所

• 王志通
• 中国科学院武汉病毒研究所

• 简浩然
• 中国科学院武汉病毒研究所

• 摘要：分离获得了一株对β-及γ-BHC均具有一定降解效果的Ⅱ₅-A菌株,该菌在好气条件下以BHC为唯一碳源。检测发现。它携带一个质粒。用丝裂霉素C进行的质粒消除试验表明,凡是消除了质粒的菌株,均丧失在以BHC为唯一碳源的无机盐培养基上的生长能力。该质粒经提取和纯化后,通过转化,成功地转移到大肠杆菌（Escherichia coli C600）,使该转化子获得降解BHC的能力,并在转化子中检测出与原菌株电泳泳动度相同的质粒。因此,认为Ⅱ₅-A菌株降解BHC的基因是位于它所携带的质粒上。作者暂将此降解性质粒命名为BHC质粒,并编号为pWE1。

• Abstract：Benzene hexachloride (BHC) is a notorious insecticide recalcitrant in nature and hence renders serious environmental pollution after extensive application for years. To deal with this problem, a previous work in our Institute has found a strain of bacterium II5-A being able to degrade both β-and γ-BHC. This strain can utilize BHC as a sole carbon source under aerobic conditions. It is the finding that the strain II5-A harbours a plasmid encoding the information of BHC degradation. Experiment was done as follows.Results of curing experiment with mitomycin C proved that the clones which have been cured plasmid lost the growing ability in the medium of mineral salts with BHC as sole carbon source. The curing ratio was 3-14.7 per cent. It is suggested that the plasmid existing in II5-A carries gene(s) relating to degradation of BHC.The isolated plasmid was then transferred to Escherichia coli C600 and the transformants possessed the ability to degrade BHC successfully. The plasmid from the transformant is found the same as that from II5-A in electrophoretic characterization.All of the results show the evidence that the gene coding for BHC degradation locates on the plasmid, which is thus called as BHC plasmid.This approach may give some idea how to control BHC pollution.

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