• 关键词：平阳霉素  碎裂  双微体  染色体畸变  多重断裂

• 基金项目：

• 王钦南
• 中国科学院遗传研究所

• 沈光平
• 中国科学院遗传研究所

• 宋海燕
• 中国科学院遗传研究所

• 费云标
• 中国科学院发育生物研究所

• 摘要：本文报道平阳霉素（Pingyangmycin,PYM）的三种成分A₂、A₅和A₆诱发CHO-K1细胞染色体畸变和姐妹染色单体交换（SCEs）。处理后第一次分裂中期染色体型畸变占优势,其中有大量的染色体碎裂（Disintegration）,在含染色体碎裂的细胞中三分之一伴随出现双微体（Double minutes,DMs）。细胞畸变的频率随处理浓度升高而增加。 处理后第二次分裂中期,细胞畸变的频率仍随处理试剂浓度增加而升高,SCEs频率随处理

• Abstract：The capabilities of three components of PYM, A₂, A₅ and A6 for inducing chromosome aberrations and SCEs were studied. The results indicated that all three components of PYM could induce aberrations. For the 1st metaphase after treatment, over 60% of the aberrated were chromosome type aberrations. The chromosome disintegration, one third of which was accompanied with double minutes, was also observed. In the concentration range of 0.2-1.0μg/ml, the aberrated cell frequencies in every treatment increased with the increase of concentration of the three components. For the 2nd metaphase after treatment, both aberrated chromosomal frequency and SCE frequency increased with the increase of the concentration. According to their capabilities for inducing chromosomal aberrations, in particular the double minutes, and enhancing SCEs, it was believed that A₂, A₅ and A6 are not only mutagens but also carcinogens.

• Key words：Pingyangmycin  disintegration  double minute  chromosomal aberration  multiple breakage

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