研究论文
韩树琴,杨惠芳.蜡状芽孢杆菌45号固定化细胞脱除酸性红B的研究[J].环境科学学报,1988,8(1):93-97
蜡状芽孢杆菌45号固定化细胞脱除酸性红B的研究
- DECOLORrzATION OF ACID RED B BY IMMOBILIZED CELLS OF BACILLIUS CEREUS No.45
- 摘要：研究并比较了蜡状芽孢杆菌45号自然细胞和固定化细胞的某些性质。结果表明,该菌株的两种细胞脱色反应的最适温度均为37℃,热稳定性良好。在50ppm的酸性红B溶液中经37℃保温处理后,两种细胞的脱色酶活性有明显提高。其脱色反应的最适pH值分别为7—10和5—10。固定化细胞在4—6℃的冰箱中保存52天,脱色酶的活性不变。应用固定化细胞连续处理酸性红B溶液,当进水浓度为42.1ppm时,出水平均脱色率可达87％。
- Abstract：The properties of immobilized and native cells of Bacillius cereus No. 45 were studied. It was demonstrated that both cells were thermal stable and the optimum temperature for enzymatic reaction was 37℃. The optimum pH of enzymatic reaction appeared to be in the range of 7-10 for native cells and 5-10 for immobilized ones. The enzymatic activity of these cells for decolorization increased remarkably during the incubation in a solution of 50ppm acid red B at 37℃. The activity of immobilized cells remained constant after 52 days of storage at 4-6℃. An immobilized cell column has been used for continuous treatment of an acid red B solution for 14 days, with an influent concentration of 42.1 ppm, the average removability was 87%.
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