研究简报
狄少杰,刘凌云.黄鳝染色体体外SCE检测系统的建立及对两种清洗剂的检测[J].环境科学学报,1988,8(2):249-254
黄鳝染色体体外SCE检测系统的建立及对两种清洗剂的检测
- AN IN VITRO SCE DETECTING SYSTEM OF CHROMOSOMES IN M. ALBUS AND ITS USE IN THE ASSAY OF MUTAGENICITY OF DETERGENTS
- 摘要：姐妹染色单体交换(SCE)技术已在细胞生物学、细胞遗传学、环境毒理学、医学等领域得到了重视和应用。在检测环境诱变剂和致癌剂方面,SCE比染色体畸变敏感二百倍。 关于体外SCE检测系统的研究,除了较多地利用人外周血淋巴细胞和中国仓鼠卵巢(CHO)细胞作为SCE检测材料外,也开始利用各种生态环境中的动物。对鱼类SCE的研究,国外做过一些工作,但有关建立SCE检测系统的报道极少。
- Abstract：The sensitivity of sister chrom atid exchanges (SCEs) of blood lymph-ocytes of M. albus was tested with different dosages of mtomycin C(MMC) and cyclophosphamide (CP), u sing SCE analysis technique. The results showed that the spontaneous frequency of SCEs was quite low (0.527 SCEs/M. P). However, the frequen cies of SCEs induced with MMC or CP increased by 1.24 to 23.37 times and 2.50 to 6.5 times respective-ly, comparing to the control group. The SCEs induced by MMC and CP in M. albus were much more sensitive than those in other animals. The technique was used to assay the mutagenicity of detergents and it was found that a cleaner and a washing powder at concentrations of 0.2 to 0.6 ug/m could causa significant iacrease in frequency of SCEs.
- Key words：SCE detecting system fish detergent.
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