研究论文
张素琴,陈延,赵姬勇,王志通.假单胞菌P12对菲的降解[J].环境科学学报,1989,9(1):80-85
假单胞菌P12对菲的降解
- DEGRADATION OF PHENANTHRENEBY PSEUDOMONAS SP. P12
- 基金项目：国家自然科学基金项目
- 作者
- 单位
- 张素琴
- 中国科学院武汉病毒研究所
- 陈延
- 中国科学院武汉病毒研究所
- 赵姬勇
- 中国科学院武汉病毒研究所
- 王志通
- 中国科学院武汉病毒研究所
- 摘要：从武汉石油化工厂的活性污泥中,分离到一株可以以菲为唯一碳源和能源的假单胞菌P12该菌经靛蓝产生法测定有双加氧酶,在菲无机盐培养基中生长时,有3-菲酚物质积累,经检测有一条质粒带。用丝裂霉素C消除质粒后的菌落,失去了降解菲的能力,说明P12对菲的降解功能是由质粒控制的。
- Abstract：An organism, identified as Pseudomonas sp. P12 was isolated from an enrichment culture,in which phenanthrene was served as sole carbon and energy sources. Dioxygenase was detected in the bacterium by the method of indigo production.There was an accumulation of 3-phenanthrol during the growth of the cells in phenanthrene medium. Using gel electrophore-sis technique, the organism was found to harbour a plasmid and they could fail to grow on phenanthrene plate with curing of plasmid, indicating that the plasmid was highly related to the degradation of phenanthrene.
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