研究论文
吴云,马国华,朱湘民,吴仁慧,张学江,吴生堂.降解酚的根瘤菌的研究[J].环境科学学报,1989,9(2):183-187
降解酚的根瘤菌的研究
- A STUDY ON PHENOLS-DEGRADING RHIZOBIA
- 基金项目：
- 作者
- 单位
- 吴云
- 中国科学院武汉病毒研究所
- 马国华
- 中国科学院武汉病毒研究所
- 朱湘民
- 中国科学院武汉病毒研究所
- 吴仁慧
- 中国科学院武汉病毒研究所
- 张学江
- 中国农业科学院油料作物研究所
- 吴生堂
- 中国农业科学院油料作物研究所
- 摘要：对两铢以铺代谢方式降解酚和离体固氮的根瘤菌(代号为:101-3,97-1)的特性进行了研究,其耐酚能力在1200ppm内,五天内能在完全培养基中把300ppm苯酚降解完;在含酚培养基中多次传代后,生长周期加快、产气,而结瘤特性丧失,但离体固氮酶活性比原菌株略有增加。经60ppm的吖啶橙处理后,获得的无质粒菌株,其降解酚的能力和结瘤特性完全消失,TTC(3-苯基-4-氮唑化氯)还原性增加,而革兰氏染色反应、运功性不变。
- Abstract：Two strains of phenols-degrading free-living cometabolism Rhizobia (R. japanicum 191-3 and R. cowpea 97-1) were isolated and the strains could degrade phenols up to 300 ppm in complete medium within 5 days. After transfered and incubated several times in phenols medium, the strains shortened their life cycle, produced gas and eliminated nodulation, but their nitrogenase activity increased slightly. By treated with acridine orange solution of 60 ppm, the phenols-degrading function and the ability of nodulation of the strains were eliminated totally, while the TTC (tri-benzen tetrazolium chloride) reducing activity was increased and the Gram reaction and movement were not changed.
- Key words：Rhizobia phenol degradation nitrogen fixation cometabo-lism
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