• 关键词：苯甲酸钠降解菌  Tn5诱变  接合子

• 基金项目：

• 谢旗
• 广东省微生物研究所, 广州

• 郭海鸿
• 广东省微生物研究所, 广州

• 简浩然
• 广东省微生物研究所, 广州

• 岑英华
• 广东省微生物研究所, 广州

• 摘要：采用富集法从柴油污染的土壤中分离到一株能以苯甲酸钠为唯一碳源而生长的产水溶性荧光色素的Pseudomonas sp,其荧光色素在121℃、30min不被破坏.通过转座子Tn5插入诱变,再次证明Tn5所携km^Rsm^R基因可在革兰氏阴性菌Pseudomonas sp中表达,其km^Rsm^R双抗突变频率为10^-6-10^-7/受体细胞,从上述双抗子中可再选出功能突变菌落,频率约0.5％.其中有2株为荧光消失型,2株为荧光增强型,1株为苯甲酸钠利用能力缺陷型,1株为营养缺陷型,2株为生长缓慢型.

• Abstract：A fluorescence Pseudomonas sp. GM1 which is capable of growth on benzoic acid as a sole source of carbon and energy was isolated by selective enrichment from oil polluted soil. This organism produces a water soluble fluorescence pigment which is stable to heat at 121 ℃ for 30 min. A random Tn5 mutagenesis was carried out by using "suicide" Tn5 vector PSUP2021. This system yielded a SmRKmR transconjugant of Pseudomonas sp. with relatively high frequency(10-6-10-7/recipient cell). Eight functionally defective mutants were obtained from 1600 SmRKmR colonies (about 0.5% frequency). The mutants include: one defective in use of benzoic acid, one auxotroph,two slow-growing, two intensively producing fluorescence pigment and two defectively producing fluorescence pigment.

• Key words：benzoic acid degradating bacterium  Tn5 mutagenesis  transconjugant  soil.

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