• 关键词：pH  酸碱平衡  血液  草鱼

• 基金项目：国家“七五”攻关项目

• 余日清
• 中国科学院水生生物研究所, 武汉

• 贺锡勤
• 中国科学院水生生物研究所, 武汉

• 摘要：研究了低pH（6.0—4.0）对草鱼血液酸碱平衡、p_o2及p_co2的影响,结果表明,低pH引起草鱼严重的酸血症.亚致死pH（6.0—5.0）时,血液酸碱平衡的影响主要表现为碱贮备[HCO₃]的丧失,血液pH的明显下降经机体缓冲调节可趋于稳定.致死低pH（4.0）时,血液pN和[HCO₃]下降均非常显著,并在96h内随酸化时间延长而日趋严重.仅在pH≤4.0的酸水中草鱼存在低氧症影响问题.草鱼是一种酸敏感性鱼类,为确保成

• Abstract：Blood acid-base balance and partial pressure of O₂ and CO₂ were studied in grass carp (Ctenopharyngodon idellus) exposed to low environmental pH (6.0-4.0). The results showed that the fish suffered from severe acid aemia. At sublethal pH 6.0-5.0, the effect on blood acid-base balance appeared mainly on loss of blood bicarborate [HCO^3-] and apparent decrease in blood pH could be stabilized by buffer-regulating in organism.Other than a more substantial blood acid-base disturbance, anoxia also occured in grass carp at pH 4.0 for 96 h. It is suggested that grass carp is relatively-acid sensitive. In order to protect the survival and propagation of adult fish in natural water, pH should be kept above 6.0.

• Key words：low pH  acid-base balance  blood  grass carp.

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