研究论文
沈建伟,竺乃恺,金祖亮,徐晓白.2-硝基芴在大鼠肝匀浆S-9组分作用下的还原代谢[J].环境科学学报,1992,12(2):209-215
2-硝基芴在大鼠肝匀浆S-9组分作用下的还原代谢
- REDUCTIVE METABOLISM OF ENVIRONMENTAL CARCINOGEN 2-NITRO-FLUORENE BY RAT LIVER S-9 FRACTIONS
- 基金项目：国家自然科学基金;;中国科学院基金项目
- 作者
- 单位
- 沈建伟
- 中国科学院生态环境研究中心, 北京 100085
- 竺乃恺
- 中国科学院生态环境研究中心, 北京 100085
- 金祖亮
- 中国科学院生态环境研究中心, 北京 100085
- 徐晓白
- 中国科学院生态环境研究中心, 北京 100085
- 摘要：初步研究了在氮气保护无氧条件下,经Aroclor1254诱导的大鼠肝微粒体酶对2-硝基芴的还原代谢过程.对代谢体系及条件进行了选择用HPLC对代谢产物进行分离研究,经保留时间及紫外光谱核对显示2-硝基芴的主要还原代谢产物为2-氨基芴,除此之外还有三个量少的其代谢产物形成,因含量低而尚未进行结构鉴定.
- Abstract：Nitro polycyclic aromatic hydrocarbons such as 1-nitro-pyrene, 2-nitro-fluorene, 6-nitro-benzo[a]pyrene and 4-nitro-biphenyl have been proved to be carcinogenic in mammalian assays. The mechanismof carcinogenesis is thought to be mediated by the metabolic reduction of these nitro compounds to reactive hydroxylamine intermediates by mammalian liver, lung,nasal tissue and intestinal microflo-ra.In this paper reductive metabolism of 2-nitro-fluorene by rat liver enzyme induced by Aroclor 1254 has been investigated under nitrogen atmosphere. The metabolites have been isolated by reversed phase HPLC and identified by comparison of their chromatogra-phic and UV spectral properties with the authentic compounds. The results show that the reductive metabolism of 2-nitro-fluorene yields mainly 2-amino-fluorene (a well-known carcinogen) plus threeminor products, which remain unidentified because of insufficientquantities. The results of this study also confirm the complexity of the metabolic activation pathways.
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