研究简报
刘淑芬,Rappaport S M,Bochell W J.用增强灵敏度的~32P后标记法测定DNA-环氧苯乙烯加合物[J].环境科学学报,1992,12(2):254-259
用增强灵敏度的~32P后标记法测定DNA-环氧苯乙烯加合物
- DETECTION OF STYRENE OXIDE-DNA ADDUCTS BY P1-NUCLEASE-MEDIATED ENHANCEMENT OF SENSITIVITY OF 32P1 -POST-LABELING IN VITRO
- 基金项目：
- 作者
- 单位
- 刘淑芬
- 中国科学院生态环境研究中心, 北京 100085
- Rappaport S M
- 美国加州大学柏克利分校, 美国加州大学旧金山分校
- Bochell W J
- 美国加州大学柏克利分校, 美国加州大学旧金山分校
- 摘要：DNA加合物是具有亲电性化学致癌物与DNA形成共价相联的化合物,是化学致癌物损伤DNA的主要形式,能够灵敏准确地分析DNA加合物的方法是研究DNA损伤.
- Abstract：Reaction of DNA-styrene oxide produced six adducts as determined by P1-nuclease mediatedi enhancemet of sensitivity of 32P-post-labeling in vitro. In this procedure, nuclease P1 cleaves deoxyribo-nucleoside 3'-monophosphates of normal nucleotides to deoxy-ribo-nucleosides which do not serve as substrate for polynucleotide ki-nase,while most adduct nucleotides were found to be totally or partially resistant to the 3'-dephosphorylating action of nuclease P1. Five adducts were detected quantitatively with a 150 to 800 fold increase in 32P-count rates as compared with the standard procedure (without P1 nuclease).
- Key words：DNA adduct styrene oxide ~32P-post-labeling
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