• 关键词：长春花(Croseus)  细胞培养  硒酸钠  亚硒酸钠  无机汞  拮抗作用

• 基金项目：

• 朱利中
• 杭州大学化学系,310028

• Cullen W R
• UBC,Canada V6T 1Z1

• 摘要：研究了硒化合物对长春花(C.roseus)细胞悬浮生长的影响.在pH5.5培养介质中,当Se(Ⅵ)>0.5ppm或Se(Ⅳ)>0.6ppm时,将抑制C.roseus细胞悬浮生长,其致死浓度分别是100ppm Se(Ⅵ)和2.0ppm Se(Ⅳ).C.roseus对硒化合物有效强的富集作用.硒化合物的毒性和培养介质的pH值有关.在本试验条件下,硒酸钠和亚硒酸钠对Hg²⁺无拮抗作用.

• Abstract：The effects of selenium compounds on growth of Catharanthus roseus (C. roseus) cell suspension culture and effects of pH on the selenium toxicity have been investigated. Cell growth and selenium uptake were monitored. Selenate (>0.5ppm Se) or selenite (> 0. 6ppm Se) inhibited C. roseus growth. Minimum lethal concentration (MLC) values were respectively 100 ppm Se(VI) and 2. 0 ppm Se (IV). Selenite was more toxic than selenate in the cell cultures. These compounds were enriched by C, roseus. In this plant-tissue culture, selenate and selenite had not antagonistic actions towards inorganic mercury.

• Key words：Catharanthus roseus  selenium  mercury  cell culture  antagonistic action

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