研究论文
杨法军,赵保路,忻文娟.香烟气相物质引发的脂质过氧化与粒细胞呼吸爆发关系的ESR研究[J].环境科学学报,1993,13(3):355-359
香烟气相物质引发的脂质过氧化与粒细胞呼吸爆发关系的ESR研究
- STUDIES ON THE RELATIONSHIP BETWEEN LIPID PEROXIDATION INDUCED BY GAS-PHASE OF CIGA-RETTE SMOKE AND RESPIRATORY BURST OF GRANULOCYTES
- 基金项目：国家自然而科学基金资助课题
- 作者
- 单位
- 杨法军
- 中国科学院生物物理研究所,北京 100101
- 赵保路
- 中国科学院生物物理研究所,北京 100101
- 忻文娟
- 中国科学院生物物理研究所,北京 100101
- 摘要：香烟气相物质和空气都能使卵磷脂脂质体发生脂质过氧化.用ESR自旋捕集技术,以DMPO为捕捉剂,研究这种过氧化的脂质体对大鼠腹腔粒细胞(RPG)呼吸爆发的影响.结果表明,一定过氧化程度的脂质可增加促癌剂PMA刺激RPG产生的O2-.脂浓度为0.5mg/ml时,这种作用随充烟量的增加先不断增强,30s左右达最大值,然后又逐渐减小.空气引发的脂质过氧化对RPG呼吸爆发有相似的作用.
- Abstract：The effect of the peroxidized liposomes on the respiratory burst of rat peritoneal granulocytes (PRGs) was investigated by using ESR spin trapping technique with DMPO (5,5-dimethyl-pyrrolinelN-oxide). The results showed that the liposomes at a proper degree of lipid peroxidation was able to increase the production of superoxide anions by PRGs under the stimulation of PMA (phorbol 12-myristate 13-acetate). Liposomes with the concentration of 0.5mg/ml firstly gave that effect going up with the smoke bubbling time, and the maximum was reached after about 30 seconds of smoke bubbling, then going down gradually. The air-peroxidized liposomes had similar effectson the respiratory burst of PRGs.
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