• 关键词：核仁纤维区  氚水（HTO）  辐射效应

• 基金项目：

• 唐丹玲
• 暨南大学生物系,广州 510632

• 齐雨藻
• 暨南大学生物系,广州 510632

• 李靖炎
• 中国科学院昆明动物研究所, 昆明 650107

• 摘要：利用电子显微镜和银染技术研究HTO对莱哈衣藻核仁纤维区的辐射效应。结果显示：分裂间期衣藻核仁纤维区为由细线样结构组成的索状结构；衣藻细胞在含低剂量HTO（370kBq/mL）培养基培养1400h以后, 间期核仁纤维区索状结构变得膨松, 体积增大；经含中剂量HTO（3700kBq/mL）培养1400h以后, 纤维区索状结构明显解体, 轮廓变得不清晰；经含高剂量HTO（37000kBq/mL）培养基培养1400h后, 纤维区索状结构完全解体, 呈现出松散的细线样结构, 同时参与rRNA合成的一种酸性蛋白明显减

• Abstract：Effect of HTO on nucleolar fibrous core is studied by using new silverstaining and electro-microscopic examination. Nucleolar fibrous core of inter-phase of Chlamydomonas reinhardtii Dangeard appears as convoluted rope- likestructure which is composed of some thin strings. After having been culturedwith low- level tritiated water(HTO) (37×10kBq/mL)for 1400 hours,convoluted rope-like structure of nucleolar fibrous core is expanded and rela-xed, volume of fibrous core increased and string-like structure becomes moreclear; After having been cultured with rniddle-level HTO(37×10²kBq/mL)for 1400 hours, rope-like structure of fibrous core is disintegrated obviously,and the contour of rope-like structure becomes more indistinctr After havingbeen cultured with high-level HTO(37×10³kBq/mL),the convoluted rope-likesturcture of fibrous core is disintegrated completely, it appears as relaxedthin string-like sturcture, and acidic proteins,which are involved in therRNA-transcription process, decrease obviously. The result shows that nucleolar fibrous core of Chlamydomonas reinhardtiiis sensitive to HTO,and the change induced by HTO is obvious.

• Key words：Uncleolar fibrous core  tritiated water(HTO)  effect of irradiation.

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