研究论文

  • 戴树桂,庄源益,陈勇生,陈广宏,陈丽侠,肖钧.两种假单孢菌中二氯酚降解酶活性及其定域研究[J].环境科学学报,1996,16(2):173-178

  • 两种假单孢菌中二氯酚降解酶活性及其定域研究
  • STUDY ON ENZYME ACTIVITY AND DISTRIBUTION IN TWO STRAINS OF BACTERIA BY DICHLOROPHENOL DEGRADATION
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  • 基金项目:高等学校博士学科点专项基金
  • 作者
  • 单位
  • 戴树桂
  • 庄源益
  • 陈勇生
  • 陈广宏
  • 陈丽侠
  • 肖钧
  • 摘要:对两种有降解二氯酚能力的假单孢菌Pseudomonassp.DCP-1和Pseudomonassp,DCP-2中二氯酚降解酶活性及定域进行了测定,结果表明该酶的活力与菌种、培养时间等因素有关,与诱导物的关系不显著;酶主要定域在膜周及膜内;酶的活力在两种菌中的水平相当;酶的比活力在CDP-1的膜周处表现出相当高的水平。
  • Abstract:Activity and distribution of enzyme produced by two strains of bacteria (Psedomonas sp.DCP-1 and Psedomonas sp. DCP-2), which can utilize dichlorophenol, have been measured.The results shown that enzyme activity is effected by bacteria and culture time, and not by inducer; the distribution of enzyme produced by the two strains of bacteria is mainly in piroplasmic and intercellular; the specific activity in piroplasmic of Pseudomonas, sp. DCP-1 is higher than that in DCP-2.
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