• 关键词：镍  锌  铝  羊角月芽藻（S．capricornutum）  酶活性  ATP

• 基金项目：国家自然科学基金

• 孔繁翔
• 南京大学环境科学与工程系, 南京 210093

• 陈颖
• 南京大学环境科学与工程系, 南京 210093

• 章敏
• 南京大学环境科学与工程系, 南京 210093

• 摘要：研究了不同浓度镍、锌、铝对常见绿藻羊角月芽藻（S.capricornutum）生长速度、蛋白质含量、ATP水平、葡萄糖-6-磷酸脱氢酶（G6PDH）、酸性磷酸酶及硝酸还原酶活性的影响。结果表明3种金属离子在所试浓度范围内对羊角月芽藻的生长速度均有抑制作用，但单位藻培养物中蛋白质含量却随着金属离子浓度的增加而增加；高浓度金属离子对酶活性有明显抑制作用；藻细胞中ATP水平随着金属离子浓度的增加而下降。提出金属离子对藻类生物产生影响的机理可能是：高浓度金属离子的存在，打破了生物最佳的各种营养元素生物可利用性的平

• Abstract：The effects of Ni, Zn, and Al at various concentrations on the growth, ATP level and enzyme activities of green alga S. capricornutum were investigated. The results showed that the growth of alge was strongly inhibited after exposed to Ni, Zn and Al, while protein synthesis seemed not to be suppressed at all the tested concentrations. It was also shown that some enzyme activities per mg protein were lower as the ion concentration was higher. ATP level was lower in the algal cells exposed to metals compared with that of the control. The mechanism of the action on enzyme activity by heavy metal ions was suggested and it is believe that the balance of the availability of nutrients for optimum algal growth was disturbed with presence of metals at high concentration. The correlation between the response of the growth and several enzyme activities to metals was discussed.

• Key words：S. capricornutum  Ni  Zn  Al  enzyme activity  ATP.

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