研究论文
王保军,刘志培,杨惠芳.单甲脒农药的微生物降解代谢研究[J].环境科学学报,1998,18(3):296-302
单甲脒农药的微生物降解代谢研究
- MICROBIALDEGRADATIONMETABOLISMOFN-2, 4-DIMETHYL -PHENYL-N'-METHYLFORMAMIDINE(DMA)
- 基金项目：国家自然科学基金
- 作者
- 单位
- 王保军
- 中国科学院微生物研究所, 北京 100080
- 刘志培
- 中国科学院微生物研究所, 北京 100080
- 杨惠芳
- 中国科学院微生物研究所, 北京 100080
- 摘要：研究了门多萨假单胞菌DR-8(Pseudomonas mendocinaDR-8)菌株对单甲脒农药的降解代谢。该菌利用单甲脒作为生长的唯一氮源。其对单甲脒的呼吸作用试验确证了该农药的生物可降解性。单甲脒对细菌生物氧化代谢关联酶活性的影响研究表明,脱氢酶对单甲脒较敏感,而NADH氧化酶则耐受性较强。单甲脒生物降解特性研究表明,DR-8菌株的单甲脒降解酶为组成酶,主要分布于细胞壁和细胞膜组分,该酶作用于单甲脒的Km值约为5-14mmol/L.经紫外吸收光谱和薄层层析等分析检测,单甲脒降解过程中形成氨和2, 4 二甲基苯胺。该菌对于单甲脒农药的降解属于共代谢作用。
- Abstract：Degradation metabolism of N-2, 4 dimethyl phenyl-N'-methylformamidine (DMA) by Pseudomonas mendocina DR-8 was studied. The strain DR-8 and its mixed culture with other bacteria were able to utilize DMA as sole nitrogen source for growth and degrading the pesticide. The result from respiration assay by intact cells of strain DR-8 for DMA also demonstrated that the pesticide was biodegradable.Effects of DMA on the activity of enzymes indicated that dehydrogenase was susceptible to DMA, but NADH oxidase was rather tolerant to DMA. Properties of the DMA degrading enzyme were studied, the enzyme was an intracellular one, mainly located in the cell wall and membrane, the Km of the enzyme for degrading DMA was 5.14 mmol/L. The results from the detection of the intermediate metabolites for degradation of DMA by ultraviolet spectrum and thin layer chromatography as well as ammonia assay showed that biodegradation products of DMA were 2, 4 dimethylaniline and ammonia. Above study showed that the degradation of DMA by Pseudomonas mendocina DR-8 was a cometabolism.
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