研究论文
金祖亮,刘淑芬,Rappaport S.M..血红蛋白-环氧苯乙烯加合物的分析[J].环境科学学报,1998,18(5):479-483
血红蛋白-环氧苯乙烯加合物的分析
- INVESTIGATION ON DNA AFB1 ADDUCTS IN VITRO AND IN VIVO BY 32P POSTLABLEING
- 基金项目：国家自然科学基金, 美国职业与健康基金
- 作者
- 单位
- 金祖亮
- 国家自然科学基金委员会, 北京 100083
- 刘淑芬
- 中国科学院生态环境研究中心, 北京 100085
- Rappaport S.M.
- U NC AT CHA PEL H ILL, U SA
- 摘要：采用环氧苯乙烯(SO)和血红蛋白中羧酸基和巯基位的加合物同时测定的方法,对大鼠(SD)血红蛋白 SO加合物进行了体外实验及腹腔注射环氧苯乙烯和苯乙烯的体内实验。结果表明在血红蛋白中所分析的3种加合物(SG, 1-PE, 2-PE)均随着SO剂量而增加,半胱氨酸加合物和羧酸加合物之比率随着各不同组的实验而不同,而2-PE对1-PE比率较为恒定,说明SO和半胱氨酸反应α位优于β位,最低检测浓度是：SG为8pmol/g(蛋白), 1-PE为5pmol/g(蛋白), 2-PE为0.6pmol/g(蛋白).
- Abstract：An assay is described for measures of adducts of styrene oxide(SO)with hemoglobin(Hb)wich have been modified at residues containing eit her carboxylic acid or sulfhydryl groups.In the first, SO carboxylic acid adducts are hydrolyzed in basic medium to liberate st yrene glycol(SG).Th en, the remain ing residues are reacted wit h a reductive catalyst(Raney nickel)to liberate the two positional isomers of SO cysteine adducts, i.e., phenylet hanol(2-PE)and ph enylethanol(1-PE).T he products are derivat ized wit h pentafluorobenzoyl chloride and measured by GC NICI M S.The assay was evaluated w ith SO adducts of Hb which had been produced in the blood of S D rats follow ing(a)in vitro modification or(b)i.p.administration of eit her SO or st yrene.Levels of the three analytes(SG, 1-PE and 2-PE)increased w ith dose in hemoglob in.Binding of SO to cyst eine residues of blood prot eins is greatly preferred at the position rather t han the position both in vit ro and in vivo. The lowest detectable adduct concent rat ions are 8 pmol/g protein for SG, 5 pmol/g protein for 1-PE, and 0.6 pmol/g protein for 2-PE.
- Key words：styrene oxide hemoglobin adduct
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