• 关键词：DNA加合物  ³²P后标记  黄曲霉素

• 基金项目：国家自然科学基金

• 刘淑芬
• 中国科学院生态环境研究中心, 北京 100085

• 蒋湘宁
• 中国科学院生态环境研究中心, 北京 100085

• 徐晓白
• 中国科学院生态环境研究中心, 北京 100085

• 摘要：用³²P后标记的方法测小牛胸腺DNA和黄曲霉素B1体外反应产物里的DNA-AFB加合物,发现有4种不同的DNA加合物,加合物含量的RAL=1.08(加合物)/10⁷(正常核酸), 对比用DNA的4种碱基和AFB反应所形成的加合物,发现有3种DNA-AFB的加合物是来自鸟嘌呤碱基被修饰所形成的,占DNA加合物总量的90%.用定量的黄曲霉素B1腹腔注射入大白鼠中,24h取其肝、肾、肺、脾等组织,加合物的总含量分别是肝>肾>肺>脾。用AROCLOR诱导能促进各组织中DNA加合物的形成,但是对不同的组织有不同的促进作用。

• Abstract：DNA-AFB1 adducts in the samples reacted with DNA and AFB1 (in vitro) and in various organs of SD rat treated with AFB1 have been analyzed by ³²P-postlabeling. the results showed that four DNA-AFB1 adducts were detected and RAL of the four DNA adduct were (6.17—44.86) adduct/10⁹ normal nucleotide. The primary site of DNA modification was deoxyguanosine in vitro. Some DNA-AFB1 adducts were detected in organs of rat (live, kidney, lung, spleen) and extent of DNA adduxts formation in the organs was liver>kidney>lung>spleen.

• Key words：DNA adduct  ³²P2post labeling  AFB1

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