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研究论文

Isolation and characterization of a bacterial strain for the degradation of aniline

摘要：从活性污泥中分离到一株细菌AN3,能以苯胺为唯一碳源、氮源和能源生长。经鉴定为食酸丛毛胞菌(Comamonasacidovorans).该菌株可以在高达5000mg/L以上的苯胺中生长。当苯胺浓度为2000mg/L左右时,经3天培养即可全部被降解。该菌株还可以利用乙酰苯胺,但不能利用其他取代类苯胺化合物。AN3菌的生长和降解苯胺的最适温度为30℃和pH为7.0.9种金属离子对该菌的生长和苯胺的降解均有不同程度抑制作用,尤以Ag⁺和Hg²⁺为明显。该菌与苯胺降解代谢有关酶类的测定结果表明,该菌含有的邻苯二酚-2,3-加双氧酶是诱导酶。

Abstract：A bacterial strain AN3, which was able to grow on aniline as sole carbon, nitrogen and energy sources, was isolated from activated sludge and identified as Comamonas acidovorans . This strain was capable of growing on aniline medium higher than 5000 mg/L, and it only took 3 days to degrade all of the aniline about 2000 mg/L. The strain AN3 could also use acetanilide as carbon, nitrogen and energy sources, but could not use other substrate aniline compounds. The optimum temperature and pH for growth and aniline degradation were 30℃ and 7.0, respectively. Heavy metal ions could inhibit the growth of AN3 and the degradation of aniline, especially Ag⁺ and Hg²⁺ . Only the cells growing on aniline contained catechol₂,3 dioxygenase, this result also suggested that catechol 2,3 dioxigenase was an inducible enzyme for AN3 strain.