• 关键词：固定化细胞  喹啉  生物降解  降解动力学

• 基金项目：国家自然科学基金(批准号:29637010)

• 韩力平
• 清华大学环境科学与工程系环境模拟与污染控制国家重点联合实验室, 北京 100084

• 王建龙
• 清华大学环境科学与工程系环境模拟与污染控制国家重点联合实验室, 北京 100084

• 刘恒
• 清华大学环境科学与工程系环境模拟与污染控制国家重点联合实验室, 北京 100084

• 施汉昌
• 清华大学环境科学与工程系环境模拟与污染控制国家重点联合实验室, 北京 100084

• 钱易
• 清华大学环境科学与工程系环境模拟与污染控制国家重点联合实验室, 北京 100084

• 摘要：从处理焦化废水的A₂/O工艺曝气池中筛选、分离得到一株能利用喹啉作为唯一碳源、氮源和能源的细菌,经鉴定为皮氏伯克霍尔德氏菌(Burkholderia pickettii)革兰氏染色阴性,杆状.采用PVA硼酸纱布复合载体法包埋固定了该菌,用电镜观察研究了该菌在固定化载体中的分布及形态.固定化细胞降解动力学试验表明,喹啉的降解符合零级反应.比较了固定化细胞与游离态细胞降解喹啉的特性,实验结果表明,游离态细胞利用喹啉的速率较快.

• Abstract：A gram negative rod-shaped aerobe identified as Burkholderia pickettii, which can use quinoline as the sole carbon, nitrogen and energy source, was isolated from the aeration tank of A²/O process for coke-plant wastewater treatment.An innovative immobilization procedure called PVA-H-3BO-3-gauze method was used for preparing immobilized cells.The carrier structure and distribution of microbial cells in the carrier were investigated by electronic microscope.Kinetic study showed that quinoline biodegradation by immobilized cells conformed to zero-order reaction model.It was demonstrated that quinoline biodegradation by free cells was more quickly than by immobilized cells.

• Key words：immobilized cells  quinoline  biodegradation  kinetics

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