• 关键词：Cu²⁺吸附容量  细菌生长阶段  Pseudomonasputida5-x  透视电子显微镜(TEM)

• 基金项目：同济大学污染控制与资源化研究国家重点实验室访问学者基金资助

• 王磊
• 复旦大学生命科学学院,上海 200433

• 范立梅
• 浙江大学生物科学技术系

• 周琪
• 同济大学污染控制与资源化研究国家重点实验室

• 张伯生
• 复旦大学生命科学学院,上海 200433

• 李宗林
• 复旦大学生命科学学院,上海 200433

• 任大明
• 复旦大学生命科学学院,上海 200433

• 摘要：研究了不同生长阶段的Cu²⁺吸附细菌Pseudomonasputida5-x对Cu²⁺的吸附容量.结果表明在低硫培养基中,当Pputida5-x细胞生长刚进入对数生长期时,单位细胞对Cu²⁺的吸附溶量最低.而当生长进入稳定生长期的后期或死亡期的早期时,单位细胞对Cu²⁺的吸附量最大.实验结果还表明,高浓度的Cu²⁺对对数期细胞的吸附能力有一定的抑制,而对死亡期的细胞影响较小.早期研究

• Abstract：Biosorption using microorganism as biosorbent for the removal of heavy metal ions in industrial waste effluent is a useful method The biosorption capacity to Cu²⁺ of P putida 5 x harvested in different growth phase was investigated The experimental results illustrated that the Cu²⁺ adsorption capacity of P putida 5-x cultured in sulphate limiting medium (SLM) reached minimum in log growth phase, and reached maximum in late stationary growth phase to early death phase Higher concentration Cu²⁺ inhibited adsorption capacity of early cell to Cu²⁺, but no obvious affection to late cell The results of TEM analysis implied that Cu²⁺ was mainly accumulated on the cell surface, and late period cells adsorbed more Cu²⁺ on cell surface than early period cells due to late cell has more active groups of Cu²⁺ adsorption on cell surface than early cell.

• Key words：Cu²⁺ adsorption capacity  growth phase  pseudomonas putida 5-x  transmission electron microscope

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