• 关键词：蛋白质重金属结合体  稳定性  水稻籽实  蒸煮  消化酶

• 基金项目：国家自然科学基金(49771069)资助

• 何孟常
• 北京师范大学环境科学研究所环境模拟与污染控制国家重点联合实验室,北京 100875

• 杨居荣
• 北京师范大学环境科学研究所环境模拟与污染控制国家重点联合实验室,北京 100875

• 摘要：通过凝胶层析方法,对水稻籽实中的蛋白质Cd、Pb结合形态特征及其稳定性进行了研究.结果表明:水稻籽实TrisHCl提取液经SephadexG-75分离,洗脱液得到3个紫外吸收峰,在第1、3峰处出现了Cd、Pb浓度峰,其蛋白质Cd、Pb结合体的表观分子量为54.5KD和5.5KD.在蒸煮加热处理后,水稻籽实中蛋白质Cd、Pb结合体发生变性,使分子量为54.5KD的蛋白质Cd、Pb结合体完全破坏,分子量为5.5KD的结合体也部分地被破坏,与蛋白质结合的Cd、Pb被释放出来.胃蛋白酶和胰蛋白酶消化处理后,也能够

• Abstract：The protein binding forms of Cd and Pb in polluted rice seed and their tability were studied using Sephadex chromatography. Three peaks of absorption for Tris HCl extraction from rice on Sephadex G-75 were detected. The Cd and Pb distributed mainly in the fractions of F-Ⅰ and F Ⅲ. The apparent molecular weights of proteins Cd and Pb for F-Ⅰ and F Ⅲ were 54.5 and 5.5KD, respectively. After the treatment of cooking, the protein binding forms of Cd and Pb were destroyed, high molecular weight protein binding form (54.5KD) was decomposed into low molecular weight complex (5.5KD) or tiny peptide chain. Simultaneously, bound Cd and Pb were released or from protein changed into small protein binding forms. After the treatment of enzyme digestion, the high molecular weight protein binding forms of Cd and Pb were decomposed the concentrations of Cd amd Pb in the elutions of first and third peak decreased markedly, and the Cd and Pb appeared in the elution of third peak later.

• Key words：protein binding forms of heavy metals  stability  rice seed  cooking  digestive enzyme

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