• 关键词：SO₂衍生物  大蒜  微核  双核  核碎解  核固缩

• 基金项目：国家自然科学基金(39770634);山西省自然科学基金资助

• 仪慧兰
• 山西大学环境医学与毒理学研究所生命科学系,太原 030006

• 孟紫强
• 山西大学环境医学与毒理学研究所生命科学系,太原 030006

• 摘要：研究SO₂体内衍生物---亚硫酸钠和亚硫酸氢钠混合液(3:1,mol.L^-1mol.L^-1)对大蒜幼根细胞的遗传损伤效应.结果表明:SO₂衍生物处理可导致根尖细胞分裂异常,中期染色体粘连、间期细胞微核和双核频率明显增高;此外还可引起根尖部分细胞核碎解及核固缩.SO₂体内衍生物的上述效应具有明显的时间效应和剂量效应关系.

• Abstract：The cytogenetic damages of A.sativum root cells induced by the derivative of SO₂-sodium bisulfite were investigated. Abnormal division and chromosomal stickiness were observed, and the frequencies of micronuclei and binucleated cells increased significantly in the root cells when A.sativum seedlings incubated in sodium bisulfite at high concentration or at low concentration for long duration time. Furthermore, root cells with nuclear fragmentation and pycnosis were also observed at the same time. The effect of SO₂ derivatives was dependent on the concentration and treated time. It is suggested that A.sativum root cells can be used as the material to monitor SO₂ pollutant in air environment.

• Key words：sodium bisulfite  sulfur dioxide  Allium sativum  micronuclei  binucleated cell  nuclear fragmentation  pycnosis

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