研究论文
王悠,杨震,唐学玺,刘泳,李永祺.7种海洋微藻对UV-B辐射的敏感性差异分析[J].环境科学学报,2002,22(2):225-230
7种海洋微藻对UV-B辐射的敏感性差异分析
- The sensitivity variability of seven species of marine microalgae to the influence of UV-B radiation
- 基金项目：国家自然科学基金资助项目(编号:39870146)
- 作者
- 单位
- 王悠
- 青岛海洋大学生命科学学院,青岛 266003
- 杨震
- 青岛海洋大学生命科学学院,青岛 266003
- 唐学玺
- 青岛海洋大学生命科学学院,青岛 266003
- 刘泳
- 青岛市海泊河污水处理厂,青岛 266021
- 李永祺
- 青岛海洋大学生命科学学院,青岛 266003
- 摘要：在实验室条件下研究了UVB辐射对7种海洋微藻生长的影响.结果表明:7种微藻的相对增长率随着UVB辐射剂量的增强呈明显的下降趋势(P<005).实验微藻对UVB辐射的敏感性符合绿藻>硅藻>金藻的分类学规律,其中金藻870.1是最敏感的一种.叶绿素a和类胡萝卜素的48h半数有效抑制浓度EC50远远大于相对增长率的EC50.分光光度法测定微藻在UV波段的吸收峰集中在UV/C波段.在UVB波段,每10.4个细胞的最大吸收度的顺序为:绿藻>硅藻>金藻.微藻对UV/B辐射的敏感性可能与其在该波段的吸收度之间存在一致性关系.
- Abstract：Acute toxic experiments to 7 species of marine unicellular algae were performed under laboratory conditions.Three phenomena were observed:The relative growth rate(K) in treated groups provoked significant decrease(P <0.05)compared with the controlled groups. Chlorophyta and Bacillariophyta were more resistant while Chrysophyta was vulnerable in response to UV/B radiation and Isochrysis galbama Park 8701 was the most UV B sensitive species.The values of 48h chl.a EC50 and 48h car.EC50 were much higher than that of 48h/K (EC50),predicting that K was the most sensitive indicator among the three indexes.Spectrophotometric method was used to investigate the absorption in UV protion.Results showed that the absorption peaks concentrated in UV/C spectrum.The order of absorbency per 104 cells from low to high in UV/B portion was Chlorophyta>Bacillariophyta>Chrysophyta,which was concordant with the sensitivity order.The results also indicated a rough relationship between sensitivity and UV absorbency.
- Key words：UV B radiation marine icroalga sensitivity absorbency
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