研究论文
王磊,周琪,张伯生,李宗林,任大明.1,3-二氯苯降解细菌Bacillus cereus PF-11的遗传选育及其降解特性研究[J].环境科学学报,2002,22(2):231-235
1,3-二氯苯降解细菌Bacillus cereus PF-11的遗传选育及其降解特性研究
- Effective biodegradation of 1,3-dichlorobenzene by a mutant of Bacillus cereus PF-11
- 关键词：生物降解 1,3二氯苯 Bacilluscereus 氯离子 遗传突变
- 基金项目：同济大学污染控制与资源化研究国家重点实验室访问学者基金资助
- 作者
- 单位
- 王磊
- 复旦大学生命科学学院,上海 200433
- 周琪
- 同济大学污染控制与资源化国家重点实验室,上海 200092
- 张伯生
- 复旦大学生命科学学院,上海 200433
- 李宗林
- 复旦大学生命科学学院,上海 200433
- 任大明
- 复旦大学生命科学学院,上海 200433
- 摘要：以焦化厂废水中分离得到的一些苯降解细菌为出发菌,通过遗传突变方法获得了一株高效降解1,3二氯苯的突变株PF11.在合适条件下突变株PF11培养液对1,3二氯苯的降解速率可达31mg(L·d),并伴随着有效的氯离子释放.经初步鉴定,突变株PF11为以前未见报导的革兰氏阳性Bacilluscercus菌.菌株PF11的底物专一性较低,对单氯苯和1,2二氯苯均有降解作用,降解能力为单氯苯>1,3二氯苯>1,2二氯苯.初步推测最初反应步骤是双加氧酶催化的分子氧攻击苯环形成二氯邻苯二酚.
- Abstract：an effective 1,3 dichlorobenzene degrading strain PF 11 from benzene utilizing bacteria isolated from town gas industrial effluent by genetic breeding was introduced. A degradation rate of 31mg/(L·d) of 1,3 DCB could be obtained by strain PF 11 in suitable condition with effective chlorion release. Mutant PF 11 was tentatively identified as gram positive Bacillus cereus, which has not been reported in detail. The substrate specificity of mutant PF 11 was relatively low, and the degradation ability to different chlorobenzene was in the order of MCB> 1,3DCB >1,2DCB with initial attacking by molecular oxygen catalysed by dioxygenase.
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