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研究论文

Some detoxication mechanisms of different wheat varieties under cadmium treatment

基金项目：云南省应用基础研究基金(97C010M);国家自然科学基金(39970142);教育部高等学校骨干教师资助计划(GG180106732001);云南省高等学校大型精密仪器设备分析测试基金

摘要：在外源Cd²⁺处理梯度下,综合探讨细胞壁的阻隔作用、细胞活性氧防御酶系作用、植物细胞的区间隔离作用及有机酸生成等几种解毒机制对抗性的贡献.结果表明:(1)细胞壁及残渣、含核糖核蛋白体的细胞溶质部分是结合镉的主要部位,细胞核、叶绿体和线粒体结合镉量较少;(2)解毒功能强的品种并非都是对镉吸收量少的品种,吸收量少的品种也并不意味着就有较强的解毒功能,解毒是多种机制综合作用的结果;(3)POD在Cd²⁺处理下小麦细胞活性氧防御机制中起关键作用;(4)低浓度Cd

Abstract：Some detoxication mechanisms, which induded separation of cell wall, defensive enzymes of active oxygen, compartmentalization of plant cell, production of organic acid, et al.of different wheat varieties under Cd treatment, had been studied. The results showed that (1) Cadmium distributed mainly in cell wall and cytosol but little in nuclear, chloroplast and mitochondrion. (2) Not all the wheat varieties, some of which had strong detoxication function, absorbed little cadmium. Similarly, the varieties which absorbed only a little cadmium did not have a strong detoxication function absolutely. (3) POD played an important role in the defensive mechanisms of active oxygen. (4) The organic acid content of five wheat varieties had little change under low cadmium concentration. As the concentration of cadmium increascd, the organic acid content increased accordingly. (5) FM27 and FM29 had strong detoxication function while FM24 and FM25 had middle and M variety had weak detoxication function.