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专论

The effect of Dinoflagellate Alexandrium tamarense on juvenile Perch Lateolabrax japonicus

基金项目：国家重点基础研究项目(2001CB409700);国家自然科学基金项目(49906007, 39950001, 20177023, 40076030);中国科学院知识创新工程项目(KZCX2206);院长基金;山东省科技攻关项目(012110115)资助

摘要：塔玛亚历山大藻(Alexandriumtamarense)(ATHK)藻液能对鲈鱼(Lateolabraxjaponicus)幼鱼(2.5cm)的存活有明显的影响, 96h的半致死浓度为4000cellsmL.根据该藻PSP产毒量374.8fmolcell(毒性为11pg(STXeq)cell)计算, 鲈鱼幼鱼的96h半致死藻毒素浓度相当于149.92fmol/L(毒性为4.4pg(STXeq)L).通过对塔玛亚历山大藻藻液各组分(都相当于8000cellsmL):藻细胞悬浮液、去藻过滤液、细胞内容物以及细胞碎片的毒性大小比较研究, 发现藻细胞的毒性作用很强, 与藻液相近, 细胞内容物也有显著影响, 其他组分无显著的毒性作用, 结果表明幼鱼摄入PSP毒素是幼鱼致死的原因.较大的幼鱼(12cm)对有毒藻不敏感, 暴露在10000cellsmL10d后, 存活率为10.0%.探讨了塔玛亚历山大藻的致毒机制和途径.

Abstract：Culture of dinoflagellate Alexandrium tamarense showed obvious impact on the survival of the juvenile Perch(2 5cm), Lateolabrax japonicus with 96hLC50=4000 cells/mL. According to the PSP content of the alga, 37 48fmol/cell (toxicity equivalent to 11pg STX eq/cell), the 96hL/C50 of the algal toxins was equivalent to 149 92fmol/L (toxicity equivalent to 44pg STX eq/L). The toxicity of different fractions of the algal culture (equivalent to 8000cells/mL):re-suspended cells, cell free medium, cell contents and cell fragments were compared. The re suspended cells showed similar strong impact as the culture on the survival of the juvenile fish, and the cell content also resulted in significant such adverse effect, however, the other fractions did not produce any obvious toxicity. The results indicated that PSP toxins ingestion is the main lethal factor to the juvenile fish mortality. It was also found that the young fish(12cm)was not sensitive to the toxic alga, which could survive during the exposure to algal cells at 10000cells/mL for ten days.