研究简报
明镇寰,岳春梅.生物硝化池污水中硝化细菌的快速定量研究[J].环境科学学报,2002,22(6):796-798
生物硝化池污水中硝化细菌的快速定量研究
- Study on fast quantitative detection for Nitrobacteria in wastewater of biological nitrification stage
- 基金项目：浙江省科技厅重点项目(001103172);浙江省环保局项目资助
- 作者
- 单位
- 明镇寰
- 浙江大学生命科学学院, 杭州 310012
- 岳春梅
- 浙江大学生命科学学院, 杭州 310012
- 摘要：实验采用聚合酶链式反应(PCR)技术与最大几率数法(MPN)相结合的MPNPCR法对生物硝化池污水中的硝化细菌进行快速定量.所用的一对PCR引物是在对硝化细菌的16SrRNA基因进行系统比较的基础上设计合成的, 可以扩增出大小为388bp的DNA片段.以从生物硝化池污水中抽提的含硝化细菌DNA的混合DNA为模板, 进行PCR扩增并确定合适的扩增条件.运用MPNPCR法进行定量检测的整个过程可在几小时之内完成.
- Abstract：The MPNPCR method, based on the combination of polymerase chain reaction with most probable number, was applied for quantitative detection of Nitrobacteria in wastewater of biological nitrification stage.The oligonucleotide primers, designed by comparing the total 16SrRNA gene sequences of Nitrobacteria, were used to generate a 388 bp DNA fragment.The mixed DNA (with the Nitrobacteria DNA from the wastewater in the biological nitrification stage) Served as template for PCR and the best amplification condition was determined.It took only several hours to count Nitrobacteria by MPNPCR procedure.By comparing the results of MPNPCR with that of MPNGriess, it was found that MPNPCR can sensitively, consistently, efficiently and rapidly assay, which is effective to detect and count the Nitrobacteria in the wastewater of the biological nitrification stage.
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