研究论文
高平平,赵义,赵立平.焦化废水处理系统微生物群落结构动态的ERIC-PCR指纹图谱分析[J].环境科学学报,2003,23(6):705-710
焦化废水处理系统微生物群落结构动态的ERIC-PCR指纹图谱分析
- Analysis of the microbial community of activated sludge in different aeration basins within an industrial phenol remediation system by ERIC-PCR fingerprinting
- 基金项目：国家高科技研究发展计划项目(“863”项目计划)(No.SZ030104,2001AA214131)
- 作者
- 单位
- 高平平
- 1. 山西大学生物技术研究所,太原 030006; 2. 上海交通大学生命科学技术学院,微生物分子生态学与基因组学实验室,上海 200240
- 赵义
- 太原理工大学环境工程系,太原 030024
- 赵立平
- 上海交通大学生命科学技术学院,微生物分子生态学与基因组学实验室,上海 200240
- 摘要：ERICPCR群落指纹图谱监测与常规技术相结合,分析了焦化废水处理系统曝气池的微生物群落结构动态变化与污染负荷和主要污染物降解效果变化的关系.在每3d1次,连续8个时间点的监测中,待处理废水中苯酚负荷在35.53mgL和132.82mgL之间波动,氰化物负荷从0.96mgL变化到34.75mgL,CODCr最低为1044.10mgL,最高时为7930.90mgL.第4监测期间高浓度矿物油冲击使活性污泥的沉降能力和对CODCr的降解效率显著降低,但对苯酚和氰化物的降解能力造成的影响较小.监测期间2个曝气池微生物群落的ERICPCR指纹图谱的平均相似性系数(Cs)分别为94.1%和96.6%,多样性指数在1.77-2.34和1.60-2.16之间,表明用ERICPCR检测出的该系统中的活性污泥这部分微生物种群的结构比较稳定,可能构成了苯酚和氰化物去除效率在监测期间相对稳定的基础.
- Abstract：The occurrence, settleability and activity of activated sludge in first (TJ1) and second (TJ2) aeration basin in a AB process based industrial phenol remediation system were analyzed by ERIC-PCR(Enterobacterial Repetitive Intergenic Consensus-PCR) fingerprinting combined with activated sludge characterization (SV30、MLSS、SVI), and major pollutants (phenol, cyanide and CODCr) measurements. During the monitoring period (one sampling every three days for 8 continuous samplings, M1-M8), the concentrations of primary pollutants in the influent fluctuated from 35.53mg/L to 132.82mg/L for phenol, between 0.96mg/L to 34.75mg/L for cyanide and from 1044.10mg/L to 7930.90mg/L for CODCr. The presence of a pollution shock caused by mineral oil contamination occurred at M4 monitoring stage resulted in a dramatic decrease in activated sludge settleability and CODCr removal efficiency. The introduction of this loading shock had no significant impact on the system's ability to degrade phenol and cyanide. ERIC-PCR banding patterns of activated sludge samples at 8 stages showed a mean Cs value of 94.1% within TJ1 and 96.6% within TJ2, Shannon-Weaver indices of 1.77-2.34 in TJ1 and 1.60-2.16 in TJ2. Populations in the system detected by ERIC-PCR fingerprinting were thus fairly stable. The stability of the ERIC-PCR amplifiable populations in activated sludge may serve as the basis for relatively stable removal efficiency of phenol and cyanide during the monitoring period.
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