研究论文
张培玉,唐学玺,王悠.臭氧处理对海带膜脂过氧化和脱酯化伤害研究[J].环境科学学报,2003,23(6):792-796
臭氧处理对海带膜脂过氧化和脱酯化伤害研究
- Studies on damage of membrane lipid peroxidation and deesterification caused by ozone treatment in Laminaria japonica
- 基金项目：国家重点基础研究“973”专项经费资助(G1999012004)
- 作者
- 单位
- 张培玉
- 1. 中国海洋大学海洋生态学实验室,青岛 266003; 2. 曲阜师范大学生命科学学院,曲阜 273165
- 唐学玺
- 中国海洋大学海洋生态学实验室,青岛 266003
- 王悠
- 中国海洋大学海洋生态学实验室,青岛 266003
- 摘要：采用生态毒理学和生物化学的方法,选用海带的2个品系---海带901和荣城1号为实验材料,研究了不同剂量的臭氧处理对海带膜脂过氧化和脱酯化伤害作用.结果表明,随着臭氧处理强度的增加,海带的2个品系的光合速率均下降.同时,膜相对透性增大,细胞内H2O2含量上升,微粒体膜中磷脂减少,游离脂肪酸增加.海带901中无论是细胞匀浆中丙二醛(MDA)含量,还是微粒体膜的MDA含量在臭氧处理下均无显著变化,而荣城1号微粒体膜的MDA含量在臭氧处理下显著提高,说明海带901在臭氧氧化胁迫下膜的损伤是由膜脂脱酯化单独造成的,与膜脂过氧化无关,而荣城1号膜的损伤是由膜脂过氧化和脱酯化共同引起的.
- Abstract：Changes in photosynthesis rates,membrane relative permeability,H2O2 content,membrane lipid peroxidation and deesterification in Laminaria japonica under ozone treatment were studied by ecotoxicological and biochemical method.The results showed that the photosynthesis rates decreased with the ozone treatment intensity increment in Laminaria 901 and Laminaria No.1.Membrane relative permeability and H2O2 content increased,phospholipid content decreased and free fatty acid content promoted in microsomal membrane.These meant that membrane damage was seriously induced by ozone treatment and the increase in membrane damage was relatied to the accumulation of active oxygens which initiated deesterification.However,malondiadehyde(MDA) content in Laminria 901,not only in homogenate but also in microsomal membrane,did not show significant changes,while MDA content of microsomal membrane in Laminaria No.1 exhibited obvious changes.The results suggested that the membrane damage induced with ozone treatment was due to membrane lipid deesterification in Laminaria 901,and both membrane lipid deesterification and peroxidation in Laminarina No.1.
摘要点击次数： 1721 全文下载次数： 2728
