• 关键词：黄孢原毛平革菌  液体振荡培养  氨氮浓度  木质素过氧化物酶  漆酶

• 基金项目：863计划探索性项目(2002AA649100)

• 喻国策
• 清华大学环境科学与工程系环境模拟与污染控制国家重点联合实验室,北京 100084

• 文湘华
• 清华大学环境科学与工程系环境模拟与污染控制国家重点联合实验室,北京 100084

• 李东锋
• 清华大学环境科学与工程系环境模拟与污染控制国家重点联合实验室,北京 100084

• 李锐
• 清华大学环境科学与工程系环境模拟与污染控制国家重点联合实验室,北京 100084

• 钱易
• 清华大学环境科学与工程系环境模拟与污染控制国家重点联合实验室,北京 100084

• 摘要：在氨氮浓度0.0308-0.924g/L下对黄孢原毛平革菌(Phanerochaetechrysosporium)在空气环境中进行自由悬浮振荡培养.在氨氮浓度0.0308-0.308g/L下可检测到木质素过氧化物酶活性,最高酶活为42.8U/L;在氨氮浓度0.0308g/L和0.154g/L下可检测到漆酶活性,最高酶活为35.0U/L.在氨氮浓度不低于0.154g/L时,葡萄糖消耗速率大致相当,明显高于在氨氮浓度0.0308g/L下的葡萄糖消耗速率;氨氮在葡萄糖耗尽时达到最低值,然后开始增大;木质素过氧化物酶和漆酶的活性峰与葡萄糖和氨氮的最大消耗基本对应.实验结果对木质素降解酶发酵和白腐真菌在环境工程中的直接应用具有启发意义.

• Abstract：Agitated submerged culture of Phanerochaete chrysosporium was carried out in air at ammonium nitrogen concentrations ranging from 0.0308 to 0.924g/L. Lignin peroxidase was detected within a range of nitrogen concentration from 0.0308 to 0.308g/L with the highest peak activity of 42.8U/L, and laccase between 0.0308g/L and 0.154g/L with the highest activity of 35.0U/L. At nitrogen concentrations of 0.154g/L and above, glucose consumption rates were comparable and evidently higher than that at 0.0308g/L, and ammonium nitrogen reached minimums when glucose was depleted and thereafter began to increase. Activity peaks of lignin peroxidase and laccase coincided with the maximum or nearly maximum consumption of glucose and nitrogen at nitrogen concentrations of 0.154g/L and above. The results would be of importance in the fermentation of the ligninolytic enzymes and the direct application of white rot fungi in environmental engineering.

• Key words：Phanerochaete chrysosporium  agitated submerged culture  ammonium nitrogen concentrations  lignin peroxidase  laccase

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