研究论文
王建龙,赵璇,Wener Hegemann.氯酚的生物降解特性及其微生物的16SrRNA基因序列分析[J].环境科学学报,2003,23(6):813-817
氯酚的生物降解特性及其微生物的16SrRNA基因序列分析
- Characteristics of chlorophenol degradation and the 16S rRNA gene sequencing of the strain
- 基金项目：国家自然科学基金(批准号:29637010);德国洪堡基金会(AlexandervonHumboldtStiftung)资助
- 作者
- 单位
- 王建龙
- 1. 清华大学核研院环境技术研究室,北京 100084; 2. 环境模拟与污染控制国家重点联合实验室,清华大学,北京 100084
- 赵璇
- 清华大学核研院环境技术研究室,北京 100084
- Wener Hegemann
- Technische Universitaet Berlin,Institut fuer Technische Umweltschutz,Sekr KF 7,Strasse des 17,Juni 135,D-16023,Berlin,Germany
- 摘要：从受氯代有机物污染的土壤中富集分离到对2,4-二氯酚具有高效降解能力的微生物混合菌群.实验表明,降解1mol二氯酚可以定量释放出2mol的氯离子.在生物流化床反应器中,以聚胺酯泡沫块为固定化载体吸附固定化微生物,进行了连续降解氯酚的实验研究,当水力停留时间为24h,二氯酚的初始浓度为30μmolL时,二氯酚的去除率均在90%以上.利用平板划线法从混合微生物菌群中分离到可以利用二氯酚为唯一碳源和能源的纯种微生物.16SrRNA基因序列分析结果表明,该微生物为Rhodococcus属.
- Abstract：A stable bacterial consortium capable of degrading 2,4-dichlorohenol was isolated from the soil contaminated by chlorinated organic chemicals.The batch experiment indicated that a stoichiometric releases of 2mol chloride per 1mol dichlorophenol.The continuous degradation of dichlorophenol was carried out in a biological fluidized bed reactor using polyurethane foam as immobilizing carrier.The results showed that the removal efficiency of dichlorophenol was above 90% when hydraulic retention time was 24h and the initial concentration of 2,4-dichlorophenol was 30μmol/L.From the mixed culture a pure strain capable of using 2,4-dichlorophenos as the sole carbon and energy source was isolated.According to the results of 16S rRNA sequencing the strain is affiliated to the genus Rhodococcus.
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