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研究论文

Effects of airborne fine particulate matter in Beijing on cell cycle of (Balb/c) 3T3 cell

摘要：研究了北京市大气细颗粒物有机提取物(EOC)对Balb/c3T3细胞周期及调节因子的影响.实验发现116μg/mL细颗粒物EOC即有明显的细胞毒性, 抑制细胞生长, 并存在剂量反应关系, IC50为402.2μg/mL;流式细胞仪分析显示1366μgmLPM_2.5EOC可造成细胞周期改变, G0/G1期、G2M期细胞分布增加, 分别为16.24%(P<0.01)与3.17%(P<0.05), S期细胞分布减少20.%(P<0.01), 并伴有P53含量的增加;409.8、478.1μg/mL的PM_2.55EOC可诱导Balb/c3T3细胞凋亡.提示PM_2.5有机提取物可能通过P53途径引起Balb/c3T3细胞周期阻滞, 并诱导细胞凋亡.

Abstract：The aim of this study was to investigate the effect of organic compounds （EOC） extracted from airborne fine particulate matter(PM_2.5) in Beijing on cell cycle of Balb/c 3T3 cell. Cytotoxicity of EOC from PM_2.5 was examined by MTT.The effects of EOC on cell cycle and apoptosis were observed by flow cytometry and DNA ladder.The level of P53 protein was determined using western blotting. From the results, we find that EOC in PM_2.5 inhibited the growth of Balb/c 3T3 cell line dose-dependent manner and IC₅₀ was 402.2μg(PM_2.5)/mL.EOC in PM_2.5 could disturb the cell cycle of Balb/c 3T3 cell.There were 16.24%（P<0.01）and 3.17%（P<0.05）increases in ratios of G0/G1 and G2/M, respectively and there was an incrase of 20.4%（P<0.01） in ratio of S, while the level of P53 increased in a dose-dependent manner.Apoptosis could be induced by EOC in PM_2.5 at 409.8 and 478.1μg(PM_2.5)/mL.The data suggest that EOC in PM_2.5 could disturb the cell cycle through P53 route and induce apoptosis in Balb/c 3T3 cell.