• 关键词：微囊藻毒素  提取  提纯

• 基金项目：国家自然科学基金(编号:30270277, 20377047和20177029);973项目(2002CB412308);863项目(2002AA601120)

• 闫海
• 中国科学院生态环境研究中心, 环境水质学国家重点实验室, 北京 100085

• 潘纲
• 中国科学院生态环境研究中心, 环境水质学国家重点实验室, 北京 100085

• 张明明
• 中国科学院生态环境研究中心, 环境水质学国家重点实验室, 北京 100085

• 陈海柳
• 中国科学院生态环境研究中心, 环境水质学国家重点实验室, 北京 100085

• 邹华
• 中国科学院生态环境研究中心, 环境水质学国家重点实验室, 北京 100085

• 摘要：对采自云南滇池水华蓝藻细胞中的微囊藻毒素(Microcystins, MCs)的提取与提纯方法进行了研究.结果表明, 在藻细胞干重浓度为20g·L^-1下, 与不同甲醇浓度提取液相比, 40%甲醇溶液可以最有效地从藻细胞中提取出MC/RR和MC/LR.将MCs提取液过Waters固相萃取小柱后, 用70%甲醇溶液洗脱吸附于柱上的MCs, 可以分别获得73%和35%纯度的MC/RR和MCLR.通过观察洗脱液的颜色变化, 收集蓝绿色和橘黄色后面流出的基本无色的洗脱液, 可以获得纯度为28.6%和12.9%的MC/RR和MC/LR.

• Abstract：The methods for extracting and purifying microcystin (MCs) in dry cyanobacterial cells taken from Dianchi Lake, Yunnan Province, were studied in this paper. 40% methanol-water solution was found to be an optimum solution for effectively extracting MC-RR and MC-LR from cyanobacterial cells among different ratios of methanol when the concentration of dry cyanobacterial cells was 20g·L^-1. After the extraction solution of 40% methanol containing MCs was passed through a cartridge (Waters, OASIS~(TM) HLB Cartridge), MCs were purified by the elution with 70% methanol, and 7.3% and 3.5% purities of MC-RR and MC-LR were obtained. By collecting the achromatic fraction of the elution after blue-green and orange-yellow color elution, MC-RR and MC-LR with the purities of 28.6% and 12.9% were obtained.

• Key words：microcystins  extraction  purification

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