• 关键词：甲醇废水  产甲烷细菌  耐酸性  颗粒污泥  UASB处理工艺  间歇培养试验

• 基金项目：建设部科技攻关项目;科技成果重点推广项目(99099)

• 周雪飞
• 同济大学污染控制与资源化国家重点实验室,上海　200092

• 任南琪
• 哈尔滨工业大学市政环境工程学院,哈尔滨　150090

• 摘要：采用两段UASB处理工艺和间歇培养试验,对高浓度甲醇废水厌氧处理的运行特性进行了研究,并较深入地研究了颗粒污泥和产甲烷菌的耐酸特性及代谢活性.试验结果表明,两段UASB反应器的pH值较低,分别为4.9～5.8和5.5～6.2,显然,这不是一般产甲烷细菌适宜的pH范围.系统中的产甲烷菌即巴氏产甲烷八叠球菌,具有一定耐酸性,在pH为5.0时仍然能够降解甲醇,如果进一步培养,其耐酸性将有所提高.系统中的颗粒污泥能够保护其内部的产甲烷菌,较少地受酸性环境的影响,并在pH为4.5时仍能降解甲醇.颗粒污泥的这种性能与其结构、细菌种类和颗粒体内细菌分布情况有很大关系.

• Abstract：Two stage anaerobic system was employed to treat high concentration of methanol wastewater. The results showed the pH changed from 4.9 to 5.8 in the first UASB and 5.5 to 6.2 in the second reactor. Apparently, these were no the advisable pH levels that common methanogenic bacteria could accept. The methanogenic bacteria of the system, viz. Methanosarcina barkeri, had some acid resistance and could still degrade methanol at pH 5.0. If trained further, their acid resistance would be improved. Granular sludge of the system could protect the methanogenic bacteria within its body against the impact of acid environment and make them degrade methanol at pH 4.5. The performance of granular sludge was attributed to its structure, bacteria species and the distributing of bacterium inside the granule.

• Key words：methanol wastewater  methanogenic bacteria  acid resistance  granular sludge  UASB  batch culture experiment

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