研究论文
邵云,高士祥,杨彬,蔡邦成,王连生.HP-β-CD对不动杆菌降解高浓度硝基苯的影响[J].环境科学学报,2004,24(4):690-695
HP-β-CD对不动杆菌降解高浓度硝基苯的影响
- Effect of HP-β-CD on the biodegradation of nitrobenzene at high concentration by Acinetobacter sp.
- 基金项目：国家自然科学基金资助项目(20077013);教育部博士点青年基金资助项目(2000028423)
- 作者
- 单位
- 邵云
- 1. 南京大学环境学院,污染控制与资源化国家重点实验室,南京 210093; 2. 东南大学环境工程系,南京 210096
- 高士祥
- 南京大学环境学院,污染控制与资源化国家重点实验室,南京 210093
- 杨彬
- 南京大学环境学院,污染控制与资源化国家重点实验室,南京 210093
- 蔡邦成
- 南京大学环境学院,污染控制与资源化国家重点实验室,南京 210093
- 王连生
- 南京大学环境学院,污染控制与资源化国家重点实验室,南京 210093
- 摘要：研究了在不动杆菌降解高浓度硝基苯的过程中添加羟丙基-β-环糊精(HP-β-CD)对细菌生长、硝基苯的去除及中间产物的转化的影响.利用乙酸酐直接将硝基苯的降解样品酰基化,通过GC-MS分析定性出降解中间产物2-氨基苯酚.在适宜降解菌生长的400mg·L-1硝基苯初始浓度下,加入250和500mg·L-1HP-β-CD对生物量和硝基苯的降解基本无影响;当硝基苯初始浓度约为850mg·L-1时,加入HP-β-CD(>2000mg·L-1)显著促进了细菌的生长、硝基苯的降解和2-氨基苯酚的生物转化,并且促进程度与加入量成正比.这种促进主要是因为HP-β-CD的空腔对硝基苯和2-氨基苯酚的包合产生了脱毒的效果.当HP-β-CD的加入浓度分别为0,2000,4000mg·L-1时,降解菌对850mg·L-1硝基苯的降解都遵循一级反应动力学,降解速率常数由0.0077h-1分别增加到0.0089和0.0161h-1,当HP-β-CD的加入浓度为8000mg·L-1时,降解菌对850mg·L-1硝基苯的降解遵循零级反应动力学,其降解速率常数为16.1162mg·L-1·h-1.
- Abstract：The present study investigated the effect of Hydroxypropyl β cyclodextrin (HP-β-CD) on the cell growth, removal of nitrobenzene and the transformation of intermediates during the biodegradation process of nitrobenzene at high concentration by Acinetobacter sp. 2 aminophenol was identified as an intermediates using direct acetylation of biodegraded samples of nitrobenzene by acetic anhydride followed by GC MS analysis. When the initial concentration of nitrobenzene was about 400mg·L-1 (optimal concentration for degrading bacteria to grow vigorously), HP β CD added at 250mg·L-1 or 500mg·L-1 had no effect on the biomass and the degradation of nitrobenzene. However, the addition of HP β CD at above 2000mg·L-1, significantly enhanced the cell growth, the biodegradation of nitrobenzene and the transformation of intermediates when the initial concentration of nitrobenzene was about 850mg·L-1. The promoting effect was increased proportionally with the dosage of HP β CD, which was mainly due to the detoxification ability of HP β CD through the formation inclusion complexes with nitrobenzene and 2 aminophenol. The biodegradation kinetics of 850mg·L-1 nitrobenzene followed first order reaction when HP β CD was added at 0, 2000, 4000mg·L-1, and the kinetic constant was increased from 0.0077h-1 to 0.0089 and 0.0161h-1. However, when HP β CD was added at 8000mg·L-1, the kinetics was changed to zero order reaction and the kinetic constant was 16.1162mg·L-1·h-1.
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