研究论文
徐冬梅,刘广深,王黎明,刘维屏.重金属汞对土壤酸性磷酸酶的影响及其机理[J].环境科学学报,2004,24(5):865-870
重金属汞对土壤酸性磷酸酶的影响及其机理
- Studies on the effects and corresponding mechanism of Hg2+ on the activity of soil acid phosphatase
- 基金项目：国家重点研究发展规划(973)项目(2002CB410800)
- 作者
- 单位
- 徐冬梅
- 浙江大学环境科学研究所, 杭州 310029
- 刘广深
- 浙江大学环境科学研究所, 杭州 310029
- 王黎明
- 浙江大学环境科学研究所, 杭州 310029
- 刘维屏
- 浙江大学环境科学研究所, 杭州 310029
- 摘要：通过土壤培养试验、紫外吸收光谱和荧光光谱技术, 研究了重金属汞对土壤酸性磷酸酶催化活性、动力学性质的影响及机理.结果表明, 汞对土壤酸性磷酸酶活性、酶促反应动力学性质均具有显着影响.汞对土壤酸性磷酸酶的作用可能为非竞争与反竞争混合的抑制类型.而汞对麦芽酸性磷酸酶的抑制作用为反竞争性抑制.重金属汞通过改变土壤酸性磷酸酶分子色氨酸、酪氨酸残基的微环境致使酶活性中心的构象发生变化, 进而对酶的催化活性产生影响.
- Abstract：By fluorescence and differential UV-absorption spectra, the effects of Hg2+ on the activity and kinetic property of soil acid phosphatase were studied in soil culture under laboratory conditions, the corresponding mechanism was discussed as well. The obtained results revealed that Hg2+ had significant influences on the above characteristics of soil acid phosphatase. Data for kinetic studies suggested that the interaction between Hg2+ and soil acid phosphatase probably be mixed types of non-and anti-competitive inhibition interactions, whereas Hg2+ was an anti-competitive inhibitor of acid phosphatase from wheat germ. In addition, Hg2+ changed the microenvironment of tryptophan and tyrosine groups of soil acid phosphatase, which were active sites of the enzyme and consequently inhibited the activity of the enzyme.
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