• 关键词：Bt克螟稻  秸杆还土  厌氧微生物  细菌群落  淹水土壤

• 基金项目：国家自然科学基金资助项目(No.20177021;30070156)

• 任馨
• 浙江大学环境与资源学院, 杭州 310029

• 吴伟祥
• 浙江大学环境与资源学院, 杭州 310029

• 叶庆富
• 浙江大学核农所, 杭州 310029

• 陈英旭
• 浙江大学环境与资源学院, 杭州 310029

• Janice E.Thies
• Dept.of Crop and Soil Sciences, Cornell University, Ithaca, NY 14850

• 摘要：在实验室条件下通过秸杆还土试验比较了克螟稻Bt基因表达最高期秸杆和同一时期亲本稻秸杆的添加对淹水土壤可培养厌氧细菌数量和细菌群落组成的影响.结果表明, 与亲本对照相比, 培养初期克螟稻秸杆的添加对淹水土壤厌氧发酵性细菌、产氢产乙酸细菌、反硝化细菌和产甲烷细菌的数量产生了显着性影响, 但培养后期这种显着性差异基本消失.PCR变性梯度凝胶电泳(DGGE)指纹图谱和主成份分析(PCA)结果表明, 两种秸杆处理土壤细菌群落组成在培养的第3周和第5周达到显着性差异, 随着培养时间的延伸, 两种秸杆处理土壤间细菌群落组成的差异逐渐减小.到培养的第11周, 两种秸杆处理土壤间细菌群落组成的差异基本消失.尽管如此, 在培养的整个过程中, 秸杆处理土壤中可培养厌氧性细菌数量和土壤细菌群落的组成均与纯土对照存在显着性差异.试验结果表明, 在实验室培养的条件下, 没有观察到转Bt基因克螟稻秸杆对淹水土壤微生物明显的长期负面影响.

• Abstract：There have been few investigations on the possible effects of genetically engineered plants on the microbiota in flooded soil. We studied the influence of the transgenic rice Kemindao (KMD) straw on the culturable anaerobic bacteria and bacterial community composition in a flooded paddy soil under laboratory conditions. KMD contained a synthetic cry1Ab gene from Bacillus thuringiensis under the control of a maize ubiquitin promoter and linked in tandem with the gusA and hpt genes. The experiments were conducted in plastic pots each containing 1500μg air-dried fluvio marine yellow loamy soil amended either with 3% (w/w) Bt-transgenic rice straw, 3% (w/w) parental rice straw or no straw. Nine hundred mL sterile distilled water was added to submerge the soil. The flooded soils were then incubated in the dark at 28±1°C. Soil samples were taken from each pot at defined intervals over the incubation to assay for anaerobic bacteria and bacterial community composition. Results showed that there were only some occasional significant differences (P<0.05) in the number of anaerobic fermentative bacteria, denitrifying bacteria, hydrogen-producing acetogenic bacteria, and methanogenic bacteria between the paddy soils amended with Bt-transgenic and with non-Bt parental rice straw during the early stages of incubation. Bacterial community composition was described by denaturing gradient gel electrophoresis (DGGE) DNA fingerprints of amplified soil bacterial 16S rRNA genes. The DGGE fingerprints showed that there were apparent differences in the composition of the bacterial community between soil amended with Bt-transgenic and non-Bt parental rice straw from three weeks to five weeks of incubation, but there were few differences in bacterial community composition between two soils after eleven weeks of incubation. However, both soils with the additions of different rice straws demonstrated significant differences in the number of anaerobic microorganisms and bacterial community composition with respect to the control soil over the entire incubation. The above results indicated that, under the laboratory conditions, the Bt-straw from KMD has no long-term negative effects on a variety of culturable anaerobic bacteria and bacterial community composition of the tested flooded paddy soil.

• Key words：Bt transgenic rice  straw  anaerobic bacteria  bacterial community composition  flooded paddy soil

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