研究论文
盛下放,何琳燕,龚建勋.二株假单胞菌的疏水性及其对菲的降解能力[J].环境科学学报,2004,24(5):942-944
二株假单胞菌的疏水性及其对菲的降解能力
- The hydrophobicity of two strains of pseudomonas and their degradation of phenanthrene
- 基金项目：江苏省社会发展项目资助(BS2002044)
- 作者
- 单位
- 盛下放
- 南京农业大学生命科学学院农业部农业环境微生物工程重点开放实验室, 南京 210095
- 何琳燕
- 南京农业大学生命科学学院农业部农业环境微生物工程重点开放实验室, 南京 210095
- 龚建勋
- 南京农业大学生命科学学院农业部农业环境微生物工程重点开放实验室, 南京 210095
- 摘要：从石油污染土壤中分离纯化到2株能以菲为唯一碳源和能源的细菌GF2和GF3, 初步鉴定均为假单胞菌属(Pseudomonas)细菌.根据细菌在烃-水两相体系中的细胞数量研究了GF2和GF3菌株的表面疏水性及其在不同环境条件下的变化, 同时对GF2和GF3在液体培养条件下降解菲的效能进行了研究.结果表明, GF2和GF3表面疏水性随培养时间、温度及pH值的变化而变化, GF3的疏水性(74.8%)大于GF2(58.6%).在25℃下培养7d, GF2和GF3对菲(250mg·L-1)的降解率分别达到85.6%和100%.
- Abstract：Two bacterial strains that could utilize phenanthrene as sole carbon and energy sources were isolated from oil-contaminated soils. The two strains w e re identified as Pseudomonas based on their morphological and physio-bioch emica l characteristics. The surface hydrophobicity of the two strains and its affecti ng factors were studied based on the cell amount of bacteria in hydrocarbon-water two-phase system. The degradation of phenanthrene by GF2 and GF3 were carried out in solution. The results showed that the hydrophobicity of the two strains c hanged with the cultivation time, temperature and pH, and the surface hydrophobi city of strain GF3 (74.8%) was higher than that of strain GF2 (58.6%).The degr ad ation rate of phenanthrene (250μmg·L-1) by the strain GF2 and GF3 was 85.6% and 100% respectively under 25℃ for 7μd.
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