• 关键词：白腐菌  杂色云芝  污染物  间甲酚  生物降解  降解动力学  动力学方程

• 基金项目：华中农业大学农业部微生物重点开放实验室资助项目(AML005);河南省科技攻关项目(0324050012)

• 吴坤
• 河南农业大学生物技术与食品科学学院,郑州450002

• 徐淑霞
• 河南农业大学生物技术与食品科学学院,郑州450002

• 闵航
• 浙江大学生命科学学院,杭州310029

• 吕镇梅
• 浙江大学生命科学学院,杭州310029

• 汪伦记
• 河南农业大学生物技术与食品科学学院,郑州450002

• 陈红歌
• 河南农业大学生物技术与食品科学学院,郑州450002

• 喻子牛
• 华中农业大学农业部微生物重点开放实验室,武昌475001

• 摘要：分别进行了间甲酚浓度为0mg·L^-1, 166.7mg·L^-1和275mg·L^-13种情况下的杂色云芝实罐发酵试验, 定时测定了3种情况下菌丝干重(DC)、淀粉消耗、间甲酚降解和酶活性随时间的变化。 在无间甲酚时, 菌丝干重在188h达到最大值2600mg·L^-1; 在120h开始出现酶活, 228h达到最大值82.15U·mL^-1后, 呈现一个稳定态势, 不因衰亡期的到来而有较大幅度的变化。 在

• Abstract：A systematic research of m-cresol biodegradation dynamics by Corio lus versicolor was made. The fermentation characters by C. versicolor in 30 L fermen tor was performed and DC(dried cell), starch, m-cresol consumption, and lac case activity was determined with an 12h interval in three cases of 0mg·L^-1, 166.7m g·L^-1 and 275mg·L^-1 m-cresol. In the absence of the m-cresol, the maximum densi ty of DC was 2600mg·L^-1 at 188h, the enzyme activity appeared at 120h, reached the maximum of 82.15U/mL at 228h and then gradually remained stably during the l ater contabescence periods. In the presence of 166.7mg·L^-1m-cres ol,, the maxim um DC was 995mg·L^-1 at 204h, which was much lower (reduced to 61.73% of nom-cresol) than that in the absence of m-cresol, but the enzyme activity appeare d at 180h, the maximum was 118.36U·mL^-1 at 204h, 44.1%higher than tha t in the abse nce of m-cresol, and then was stable without obvious variation in the late r sta ge. In the presence of 275mg·L^-1 m-cresol, the maximum DC was 32 0mg·L^-1 at 37 2h, which was 12.31% of the absence of m-cresol and 32.16% of the prese nce of 1 66.7mg·L^-1 m-cresol. The enzyme activity appeared at 264h, and the maximum one w as112.8U·mL^-1 at 396h, which was similar to that at the 166.7mg· L^-1 m-cresol co ncentration, indicating that m-cresol could reduce the higher productivity of C. versicolor for laccase. The time-order model of Coriolus versicolor grow th at t hree concentrations of m-cresol above was studied and the kinetics equation s of the growth model were simulated with one order Logistic Equation. Thus, the addi tion of m-cresol doesn't change the growth kinetics model of Coriolus ver sicolor . The kinetics of the starch consumption could be described by a Logistic model, respectively in the above three cases, which indicated that m-cresol coul d, in part, replace or inhibit the consumption of the starch. The degradation kinetic s of m-cresol by C. versicolor in the fermentative liquid was also disc ussed. At m-cresol concentration of 275mg·L^-1, its degradation appeared wit h an unstabl e and slow degradation inclination. The different equations also showed that the degradation kinetics of m-cresol by C. versicolor was different at var ious concentrations.

• Key words：white-rot fugus  coriolus versicolor  pollutant  m-cresol  b iodegradation  dynamics model  kinetics equation

• 
  摘要点击次数： 1816 全文下载次数： 3198