• 关键词：有限供氧  五氯苯酚  固定化

• 基金项目：广东省自然科学基金(31430);华南理工大学制浆造纸工程国家重点实验室开放基金(200335);中国科学院纤维素化学开放研究实验室访问学者基金(K-147)

• 陈元彩
• 1. 华南理工大学制浆造纸工程国家重点实验室, 广州 510641; 2. 中国科学院广州化学研究所纤维素化学重点实验室, 广州 510650

• 蓝惠霞
• 华南理工大学制浆造纸工程国家重点实验室, 广州 510641

• 陈中豪
• 华南理工大学制浆造纸工程国家重点实验室, 广州 510641

• 摘要：将厌氧颗粒污泥和4种氯酚的高效降解菌种固定化后,通过批处理实验较系统地研究了其在有限供氧、完全厌氧及好氧条件下在初始浓度为28和87μmol·L^-1五氯苯酚(PCP)的降解过程.结果表明,完全厌氧过程尽管能有效降解低初始浓度PCP,但在高初始浓度下由于厌氧微生物本身缺乏吸收、同化其代谢产物单氯酚(MCP)和二氯酚(DCP)的能力,而导致了有毒代谢产物的积累,阻碍了其完全降解;在有限供氧条件下,通过厌氧颗粒中的厌氧菌群与好氧或兼性菌的协同作用,借助于好氧或兼性菌消除五氯苯酚厌氧过程中产生的抑制性的低氯酚等中间产物,从而使其较完全降解;与完全厌氧过程相比,混合固定化颗粒污泥在有限供氧系统具有较低的残留TOC值.厌氧好氧微生物菌群通过固定化在微环境中有效的结合在有限供氧条件下能完全彻底降解高氯代芳香化合物.

• Abstract：The degradation of pentachlorophenol(PCP) was studied in batch experiments using co-immobilized anaerobic granular sludge and four aerobic bacterial species at low and high initial PCPconcentration(28 and 87μmol·L^-1) under oxygen-limited、anaerobic and aerobic condition. The results suggested that although PCP (pentachlorophenol) could be degraded at low initial PCPconcentration with 0IHOP, while at high initial PCPconcentration, the residual PCPconcentration were correlated highly with the IHOP, the higher PCPreduction and lower intermediates were achieved by the system receiving 30 and 50IHOP. During the anaerobic condition, the intermediates MCP and DCP might exert substrate toxicity on oxygenase expressing cultures, which would inhibit oxidation of its own degradation. However in oxygen-limited condition, the high dechlorination efficiency might be due to the co-metabolism MCPand DCPby aerobic degradation bacteria; aerobic or facultative bacteria had been found to oxidize less chlorinated or dechlorinated compounds produced in synergistic association with methanogens and strictly anaerobes during fermentation due to the combination of reductive and oxidative degradation mechanisms. The oxygen-limited system also exhibited the lower residual TOCconcentration, whereas the anaerobic system exhibited higher residual TOCconcentration. These results suggested immobilization of anaerobic and aerobic microbial communities under oxygen-limited conditions might be employed as an optimal strategy to mineralize more highly chlorinated aromatic compound with low final TOCconcentrations.

• Key words：oxygen-limited condition  PCP  immobilized cell

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