环境污染治理与修复
黄丹莲,曾光明,黄国和,胡天觉.白腐菌固态发酵条件最优化及其降解植物生物质的研究[J].环境科学学报,2005,25(2):232-237
白腐菌固态发酵条件最优化及其降解植物生物质的研究
- Optimum conditions of solid-state fermentation for white-rot fungi and for It's degrading straw
- 基金项目：国家自然科学基金资助项目(No.50179011、70171055);国家高技术研究发展计划项目("863"项目)(No.2001AA644020);国家杰出青年科学基金(No.50225926);2000年教育部高等学校优秀青年教师教学科研奖励计划资助项目;高等学校博士学科点专项科研基金(No.20020532017)
- 作者
- 单位
- 黄丹莲
- 湖南大学环境科学与工程系, 长沙 410082
- 曾光明
- 湖南大学环境科学与工程系, 长沙 410082
- 黄国和
- 湖南大学环境科学与工程系, 长沙 410082
- 胡天觉
- 湖南大学环境科学与工程系, 长沙 410082
- 摘要：通过正交实验法对影响白腐菌固态发酵降解稻草木质素的5种单因素培养条件进行了优化.实验结果表明,5种因素的最佳综合水平为:菌种驯化培养8d后以0.8%(质量比)的接种量接种入发酵瓶中,于37℃培养,发酵基质的含水率为85%,同时添加0.3%的吐温80.此外,还对未经降解处理的稻草与在上述最佳发酵条件下经白腐菌降解后的稻草分别作了紫外光谱和红外光谱分析,结果表明经该菌降解后,稻草中的木质素结构被严重破坏,难降解的大分子长键烃被切断成易降解的小分子短键烃.
- Abstract：The effects of five single culture factors on lignin biodegradation ability of white-rot fungi in state-solid medium were optimized through the orthogonal experiments. The results indicated that the optimal fermentation for white-rot fungi could be reached when white-rot fungi was cultured after 8 days, 0.8% white-rot fungi (weight ratio) was inoculated, temperature was kept at 37℃, water content of the solid substrate was maintained by 85% and 0.3% Tween 80 was added respectively. In addition, the structure of the untreated straw and the treated straw degraded under the optimal fermentation condition was analyzed through ultraviolet spectroscopy and infrared spectroscopy. It could be observed that long-bond hydrocarbons of giant molecule difficult to be biodegraded were degraded into short-bond hydrocarbons of small molecule, which could be easily biodegraded. Compared with the structure of untreated straw, the structure of the treated straw was greatly altered.
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