环境毒理与生态毒理
孙立伟,曲甍甍,李兆利,陈海刚,厉以强,孔志明.人外周血淋巴细胞彗星试验检测饮用水水质毒性研究[J].环境科学学报,2005,25(3):324-328
人外周血淋巴细胞彗星试验检测饮用水水质毒性研究
- Toxicity evaluation of drinking water in human peripheral blood lymphocytes using the comet assay
- 基金项目：江苏省科技厅社会发展项目(BS2001039)
- 作者
- 单位
- 孙立伟
- 污染控制与资源化研究国家重点实验室, 南京大学环境学院, 南京 210093
- 曲甍甍
- 污染控制与资源化研究国家重点实验室, 南京大学环境学院, 南京 210093
- 李兆利
- 污染控制与资源化研究国家重点实验室, 南京大学环境学院, 南京 210093
- 陈海刚
- 污染控制与资源化研究国家重点实验室, 南京大学环境学院, 南京 210093
- 厉以强
- 污染控制与资源化研究国家重点实验室, 南京大学环境学院, 南京 210093
- 孔志明
- 污染控制与资源化研究国家重点实验室, 南京大学环境学院, 南京 210093
- 摘要：应用人外周血淋巴细胞彗星试验对无锡充山水厂、中桥水厂,常州西石桥水厂进出水及金坛市拟用水源地长荡湖湖心水样的生物毒性进行了研究.结果表明,各水样的有机浓集物均可对人外周血淋巴细胞DNA产生不同程度的损伤,存在一定的遗传毒性.试验结果与各水样水质污染状况基本一致.同时,季节变化以及常规水处理过程对水样的生物毒性也有影响.研究表明,彗星试验可有效地用于检测饮用水质有机浓集物遗传毒性.
- Abstract：The toxic potentials of organic pollutants in source and tap water samples of three waterworks (i.e.,Chongshan, Zhongqiao and Xishiqiao) and water sample of Changdang Lake were evaluated with the comet assay. The results showed that DNAdamages in human peripheral blood lymphocytes were induced after exposure to the organic extracts from all water samples and the genetic hazard also existed. The correlation of the results between the comet assay and the pollution conditions of the water sources was displayed well. Moreover, the results indicated the season alternation and the general water treatment process could affect the bio-toxicity of the water. The experimental results demonstrated that the comet assay could be successfully applied on the genotoxicity monitoring program of the water.
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