欢迎访问《环境科学学报》编辑部网站！

快速检索			高级检索

环境污染治理技术及原理

Influence of injecting time of dyes on decolorizing reactive dyes with white rot fungus under non-sterile condition

摘要：应用氮限制液体培养基(C.N=56/8.7)研究了非灭菌环境投加染料时间对白腐真菌Phanerochaete chrysosporium降解活性艳红K-2BP染料的影响.试验结果表明,纯培养2d和3d后,在非灭菌环境投加活性艳红K-2BP染料的体系脱色率与在灭菌环境投加该染料的体系基本相当,其5d脱色率在90%以上;而纯培养1d时在非灭菌环境投加染料体系的脱色率却只有81%.引起纯培养1d体系脱色率降低的主要原因是活性艳红K-2BP对白腐真菌Phanerochaete chrysosporium的生长有抑制作用和反应体系感染了酵母菌.但是,如果纯培养延长至2d以后,由于白腐真菌菌丝体已在体系内占优势,尽管在非灭菌环境下还有酵母菌侵入,但它不会占优势,从而也就不会影响白腐真菌对染料的降解作用.因此,在氮限制液体培养基中,只要白腐真菌Phanerochaete chrysosporium在反应体系占优势,就可以适当缩短纯培养时间,提前在非灭菌环境投加染料,使白腐真菌Phanerochaete chrysosporium的培养和对活性染料的脱色同步进行.

Abstract：Influence of different injecting time of dyes on decolorizing reactive brilliant red K-2BPwith white rot fungus under non-sterile condition were investigated using nitrogen limited liquid culture medium. The results showed that the decolorization under non-sterile condition was similar with sterile condition, after it was incubated for 2 or 3 days under sterile condition. And its efficiency of decolorization was above 90% at 5 days; however, The efficiency was only 81% at 5 days if white rot fungus had been incubated for 1 day under sterile condition. The reason of that decrease was because the growth of white rot fungus was suppressed by reactive brilliant red K-2BP and the reaction system was contaminated by yeast fungus. But if white rot fungus was incubated for 2 days under sterile condition, it would become predominated strain in reaction system; meanwhile, although yeast fungus still might colonize into liquid medium, it could not influence decolorization. So, in decolorizing reactive brilliant red K-2BP system with nitrogen limited liquid medium, if only white rot fungus is predominated strain in reaction system, the incubating time under sterile condition could be shortened.

Key words：white rot fungus Phanerochaete chrysosporium non-sterile condition Decolorization reactive brilliant red K-2BP