水污染治理技术及原理
曲媛媛,周集体,王竞,吕红,邢林林.溴氨酸降解菌株的分离鉴定及特性研究[J].环境科学学报,2005,25(6):785-790
溴氨酸降解菌株的分离鉴定及特性研究
- Studies on isolation, identification and characteristics of bromoamine acid-degrading bacterium
- 基金项目：教育部跨世纪人才基金
- 作者
- 单位
- 曲媛媛
- 大连理工大学环境与生命学院, 大连 116024
- 周集体
- 大连理工大学环境与生命学院, 大连 116024
- 王竞
- 大连理工大学环境与生命学院, 大连 116024
- 吕红
- 大连理工大学环境与生命学院, 大连 116024
- 邢林林
- 大连理工大学环境与生命学院, 大连 116024
- 摘要：从污泥样品中分离得到一株溴氨酸降解菌,该菌株能够以溴氨酸为唯一碳、氮源及能源生长.通过形态、生理生化特性分析以及对16SrDNA序列进行同源比较,鉴定该菌株属于鞘氨醇单胞菌属,定名为Sphingomonas xenophaga.菌株的最适降解与生长条件为:pH=70,温度30℃,转速150r·min-1,接种量8%.在最佳条件下,溴氨酸的降解率可达90%以上,菌株可耐受的溴氨酸浓度为1000mg·L-1.通过分析菌株对水杨酸、邻苯二酚及邻苯二甲酸的利用情况,推测该菌株可能通过邻苯二酚的代谢途径降解溴氨酸.
- Abstract：Astrain that had a capability to degrade bromoamine acid (BAA) was isolated from the sludge samples. The strain could use BAA as the only source of carbon, nitrogen and energy. It was identified as Sphingomonas xenophaga based on morphological and physio-biochemical characteristics and 16SrDNA sequence analysis. Under the optimal growth conditions, with pH of 7.0, temperature of 30℃, rotating rate of 150 r·min-1 and inoculation amount of 8%, BAA could be degraded in the high efficiency (more than 90%), and the strain could tolerate up to 1 000 mg·L-1 BAA. It was assumed that the strain might degrade BAA by the o-diphenol catabolic pathway, because it could utilize o-diphenol as sole carbon source.
- Key words：Bromoamine acid Biodegradation Sphingomonas
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