污染物的环境暴露与生态毒理研究
吴新国,严炜,徐靖,肖玫,张琳.细胞色素P450代谢污染物的化学模拟与优化[J].环境科学学报,2005,25(8):1096-1100
细胞色素P450代谢污染物的化学模拟与优化
- The chemical simulation and optimization of Cytochrome P450's metabolic function
- 关键词：代谢活化 化学模拟 三氯乙烯 4(对硝基苄基)吡啶
- 基金项目：国家自然科学基金(No.20377032)
- 作者
- 单位
- 吴新国
- 武汉大学资源与环境科学学院, 武汉 430079
- 严炜
- 武汉大学资源与环境科学学院, 武汉 430079
- 徐靖
- 武汉大学资源与环境科学学院, 武汉 430079
- 肖玫
- 武汉大学资源与环境科学学院, 武汉 430079
- 张琳
- 武汉大学资源与环境科学学院, 武汉 430079
- 摘要：利用Fe2+EDTAH2O2联氨混合体系模拟了生物细胞色素P450的代谢功能,选择三氯乙烯作为典型污染物与模拟体系反应,产生的活性中间体与4(对硝基苄基)吡啶(NBP)反应生成紫红色物质,用分光光度法检测.根据产生活性中间体的多少对模拟体系的组成和反应条件进行了优化,对活化机理进行了初步探讨.结果发现联氨等还原剂的存在对模拟体系的代谢活化能力有重要影响,是体系区别于Fenton试剂的重要标志.在优化条件下,低至2×10-6mol·L-1的三氯乙烯在模拟体系中可以代谢产生活性中间体,表明该体系能够较好地模拟细胞色素P450的代谢功能.
- Abstract：Aredox system consisted of Fe 2+、EDTA、H2O2 and hydrazine was established to simulate the metabolic function of Cytochrome P450 in vitro. Trichloroethylene was selected as a typical pollutant to test the metabolic function of the simulated system. The active intermediate generated by the system reacted with 4-(4-Nitrobenzyl)pyridine (NBP) to give a red-purple colored substance and measured with spectrophotometer. According to the amount of the intermediate produced, the compositions and the metabolic conditions of the simulated system were optimized, and the simulated metabolic mechanism was briefly discussed. The results showed that a proper reductant such as hydrazine was important to the metabolic ability of the system,and was the key composition which make the system different from the Fenton reagent. Under optimized conditions, as low as 2×10-6 mol·L-1 trichloroethylene could be metabolized to produce active intermediate by the simulated system, which proved that the system could simulate the metabolic function of Cytochrome P450 properly.
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