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Isolation of acid blue B-degrading Fusarium sp.HJ01 and the studies on its degradation characteristics

摘要：从膨润土中分离出一株对酸性蓝B具有降解效果的镰刀菌(Fusarium sp.)HJ01,研究了其生长特征和动力学模型模拟、降解酶的性质以及对酸性蓝B的脱色效果.结果表明:菌株生长的适宜条件为:pH范围为6~7,蔗糖为碳源,NH₄Cl为氮源.菌体生长符合改进的logistic law模型.SDS-PAGE分析HJ01菌株所产漆酶的相对分子量约为66×10³,在低氮条件下漆酶活性高达431~812U·mg^-1·s^-1.菌体培养96h后加入到含100mg·L^-1酸性蓝B的培养基中,再培养96h,酸性蓝B的脱色率达100%.紫外光谱分析表明,酸性蓝B的发色基团蒽醌环被漆酶破坏.

Abstract：An acid blue B-degrading strain (Fusarium sp.)HJ01 was isolated from bentonite. The growing characteristics and kinetics, the characteristics of enzymatic biodegradation of Fusarium sp. HJ01 were analyzed. And the effects of biomass on decolorization of acid blue Bin aqueous samples were reported. The results showed that the pHrange between 6 and 7 for growth of Fusarium sp. HJ01 was feasible, and the sucrose and NH₄Cl,is also feasible for carbon source and nitrogen source respectively. The growth kinetics of Fusarium sp. HJ01 was fitted by the modified logistic law model. The molecular weight of laccase from Fusarium sp. HJ01 was approximately 66kDanalyzed by SDS-PAGE, and laccase activity was up to from 431 U·mg^-1·s^-1 to 812 U·mg^-1·s^-1 at the condition of low nitrogen source. The Fusarium sp. HJ01 incubated for 96 h at 25 ℃ were added to the liquid medium containing acid blue B (100 mg·L^-1). And when cultured continuously for another 96 h, the decolorization rate of acid blue Bwas up to 100%. The ultraviolet-visible spectrum showed that the chromophore anthraquinone ring of acid blue Bwas broken down by laccase.