环境毒理与生态毒理
张蕴晖,丁佳玮,曹慎,阚海东.大气细颗粒物(PM2.5)对心血管内皮细胞NOS的影响[J].环境科学学报,2006,26(1):142-145
大气细颗粒物(PM2.5)对心血管内皮细胞NOS的影响
- Study on oxidation stress effects of PM2.5 on cardiovascular endothelium cells
- 基金项目：上海市自然基金项目(No.03ZR14009);上海市科学技术标准专项(No.03DZ05052)
- 作者
- 单位
- 张蕴晖
- 复旦大学公共卫生学院环境卫生教研室, 上海 200032
- 丁佳玮
- 复旦大学公共卫生学院环境卫生教研室, 上海 200032
- 曹慎
- 复旦大学公共卫生学院环境卫生教研室, 上海 200032
- 阚海东
- 复旦大学公共卫生学院环境卫生教研室, 上海 200032
- 摘要：运用体外试验方法,探讨了大气细颗粒物的心血管毒性及其可能的作用机制采集上海城区大气中的PM2.5,并以0.01、0.05、0.2、1 mg·mL-1剂量染毒大鼠心血管内皮细胞2 h后,观察PM2.5对心血管内皮细胞内总NOS,iNOS及cNOS活力等指标的影响.发现与对照组相比,0.2 mg·mL-1染毒组的心血管内皮细胞总NOS和iNOS活力显著增加,而cNOS活力显著降低,并且随着PM2.5染毒剂量的增加,存在剂量-反应关系.这一结果表明,PM2.5可通过诱导心血管内皮细胞的iNOS活性,对心血管系统产生氧化应激损伤,氧化应激作用可能是PM2.5心血管系统毒性的作用机制之一.
- Abstract：Exposure to PM2.5 was found to be significantly correlated with the increasing cardiovascular disease mortality in recent epidemiological studies. But there have had little experimental studies to explore the potential mechanism of this adverse effect of PM2.5 until now. In this study, cardiovascular endothelium cells were treated by PM2.5 collected from urban area of Shanghai, with doses of 0.01,0.05,0.2 and 1 mg·mL-1. Parameters (including activities of total NOS, iNOS and cNOS) reflecting the oxidation stress damage were measured. Results showed that the activities of total NOS and iNOS in cardiovascular endothelium cells treated with 0.2 mg mL-1 PM2.5 were significantly higher than those in control cells. Dose-response relationship was established in this study. These results meant that PM2.5 could induce the activity of iNOS in cardiovascular cells and the oxidation stress damage might be one of the mechanisms of PM2.5 effect on cardiovascular system.
- Key words：PM2.5 cardiovascular toxicity NOS
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